Fletouris D, Botsoglou N, Psomas I, Mantis A
Aristotle University of Thessaloniki, School of Veterinary Medicine, Greece.
J AOAC Int. 1996 Nov-Dec;79(6):1281-7.
A simple, rapid, and sensitive liquid chromatographic (LC) assay for quantitative screening of albendazole 2-aminosulfone, albendazole sulfoxide, oxibendazole, oxfendazole, albendazole sulfone, p-hydroxyfenbendazole, albendazole, mebendazole, fenbendazole sulfone, and fenbendazole residues in milk was developed. Samples are made basic (pH 10) and extracted with ethyl acetate. Extracts are partitioned with water, evaporated to dryness, reconstituted with mobile phase, and analyzed isocratically by ion-pair reversed-phase LC at 292 nm. Overall recoveries ranged from 79 to 100%. Linearity was excellent in the fortification range examined (5.3-200 ng/mL). Precision data, based on within- and between-days variations, suggested an overall relative standard deviation of 2.0 to 5.8%. The method was successfully used to quantitate albendazole and fenbendazole and metabolites in milk from 2 drug-treated dairy cows.
建立了一种简单、快速且灵敏的液相色谱（LC）分析法，用于定量筛查牛奶中阿苯达唑2-氨基砜、阿苯达唑亚砜、奥苯达唑、奥芬达唑、阿苯达唑砜、对羟基芬苯达唑、阿苯达唑、甲苯达唑、芬苯达唑砜和芬苯达唑的残留量。将样品调至碱性（pH 10），并用乙酸乙酯萃取。萃取液用水分配，蒸发至干，用流动相复溶，然后在292 nm处通过离子对反相液相色谱进行等度分析。总体回收率在79%至100%之间。在所检测的添加范围内（5.3 - 200 ng/mL）线性良好。基于日内和日间变化的精密度数据表明，总体相对标准偏差为2.0%至5.8%。该方法成功用于定量检测2头经药物处理的奶牛所产牛奶中阿苯达唑、芬苯达唑及其代谢物的含量。
