Naito Toyohiro, Inoue Hiroki, Kubo Takuya, Otsuka Koji
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Anal Methods. 2021 Apr 7;13(13):1559-1564. doi: 10.1039/d0ay02195d. Epub 2021 Mar 19.
Environmental water quality monitoring plays an important role in human health risk assessments for pharmaceuticals in water and pollutant source control. A new chemical detection method was developed to enhance molecular selectivity and portability by combining the molecularly imprinted technique and an electroosmotic pump (EOP), which requires only a small pump, batteries and stopwatch in principle. Selective chemical adsorption on the surface-modified EOP decreases the pumping performance of EOP due to a decrease in the surface electric charge. For proof of concept, the microfabricated EOPs with chemical surface treatment were used to investigate the effects of surface chemical change on pumping performance. The microfluidic EOP of a size of 20 mm × 20 mm × 1 mm was modified by an interval immobilization method using the template of 4-(tributylammonium-methyl)-benzyltributylammonium chloride (TBTA) and evaluated by measuring EOF. The pumping performance of the surface-modified EOP was decreased by the selective adsorption of TBTA to a two-point recognition site on the EOP surfaces. The relationships between the flow rate and the TBTA concentration were fitted to the Langmuir equation. The EOP can selectively detect the model substance even in a mixture solution with a different chemical compound. This molecular imprinted EOP does not require large and expensive instruments for driving the device and chemical detection, which can be applied to a portable analytical device for onsite analysis.
环境水质监测在水中药物的人体健康风险评估和污染物源控制中发挥着重要作用。通过结合分子印迹技术和电渗泵（EOP）开发了一种新的化学检测方法，以提高分子选择性和便携性，该方法原则上仅需一个小型泵、电池和秒表。由于表面电荷减少，表面改性的EOP上的选择性化学吸附会降低EOP的泵送性能。为了验证概念，使用经过化学表面处理的微型EOP来研究表面化学变化对泵送性能的影响。尺寸为20 mm×20 mm×1 mm的微流控EOP通过使用4-（三丁基铵-甲基）-苄基三丁基氯化铵（TBTA）模板的间隔固定方法进行改性，并通过测量电渗流进行评估。TBTA选择性吸附到EOP表面的两点识别位点上，降低了表面改性EOP的泵送性能。流速与TBTA浓度之间的关系符合朗缪尔方程。即使在含有不同化合物的混合溶液中，EOP也能选择性地检测模型物质。这种分子印迹EOP不需要大型且昂贵的仪器来驱动设备和进行化学检测，可应用于现场分析的便携式分析设备。
