College of Food Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150028, China and National Research Center of Soybean Engineering and Technology, Harbin, Heilongjiang 150027, China.
College of Food Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150028, China.
Food Funct. 2021 May 11;12(9):4176-4198. doi: 10.1039/d0fo03347b.
Herein, polyphenols were extracted from Pinus koraiensis bark and characterized. Besides, the in vitro antioxidant activity, inhibition effect on cancer cells and the activity of the immune system were investigated. The results showed that the main component of Pinus koraiensis bark was 3,5,7,3',5'-pentahydroxydihydroflavone. PKB polyphenols demonstrated a high antioxidant activity during in vitro investigation. In vivo immunological function studies on oxidatively injured mice revealed that Pinus koraiensis bark polyphenols effectively improved the survival status of irradiated mice. PKBP also increased the spleen and thymus index of mouse immunoregulatory organs. The results indicated that the phagocytic ability of mononuclear macrophages was increased. Comparing the cell distribution of the PKBP administered group and the model group, the PKBP-administered group reduced the cells arrested in the G1 phase, while the number of cells increased in the S and G2 phases. PKBP effectively protected the mouse immune system and reduced the immune suppression caused by radiation. These findings also confirmed that oxidative damaged cells induced by radiation could be repaired. PKBP had the highest inhibitory activity on colon cancer cells HT29, breast cancer cells MFC-7, gastric cancer cells BGC-823 and cervical cancer HeLa and HT29 cancer cells. PKB polyphenols could effectively induce the production of DNA-Ladder fragments and cause DNA damage in cancer cells. PKBP also blocked the cycle of cancer cells in the G2 phase, stopped cell division and induced cancer cell apoptosis. Analysis of cell apoptosis by Annexin V-FTIC/PI double staining indicated that PKBP inhibited HT29 cancer cell proliferation.
在此，从红松(Pinus koraiensis)树皮中提取多酚并对其进行了表征。此外，还研究了其体外抗氧化活性、对癌细胞的抑制作用和免疫系统活性。结果表明，红松树皮的主要成分是 3,5,7,3',5'-五羟基二氢黄酮。在体外研究中，PKB 多酚表现出较高的抗氧化活性。体内氧化损伤小鼠免疫功能研究表明，红松(Pinus koraiensis)树皮多酚能有效改善受照小鼠的生存状态。PKBP 还增加了小鼠免疫调节器官的脾脏和胸腺指数。结果表明单核巨噬细胞的吞噬能力增强。比较 PKBP 给药组和模型组的细胞分布，PKBP 给药组减少了 G1 期阻滞的细胞，而 S 和 G2 期的细胞数量增加。PKBP 能有效保护小鼠免疫系统，减轻辐射引起的免疫抑制。这些发现也证实了辐射诱导的氧化损伤细胞可以被修复。PKBP 对结肠癌细胞 HT29、乳腺癌细胞 MFC-7、胃癌细胞 BGC-823 和宫颈癌 HeLa 及 HT29 癌细胞的抑制活性最高。PKB 多酚能有效诱导 DNA-Ladder 片段的产生，并导致癌细胞 DNA 损伤。PKBP 还阻断了癌细胞在 G2 期的周期，停止细胞分裂并诱导癌细胞凋亡。用 Annexin V-FTIC/PI 双重染色法分析细胞凋亡表明，PKBP 抑制 HT29 癌细胞的增殖。
