IBMM, University of Montpellier, CNRS, ENSCM, Place Eugène Bataillon, 34095 Montpellier, France.
Sanofi Pasteur, Analytical Sciences, 1541 Avenue Marcel Mérieux, 69280 Marcy l'Etoile, France.
Anal Chem. 2021 Apr 27;93(16):6508-6515. doi: 10.1021/acs.analchem.1c00420. Epub 2021 Apr 16.
Vaccine adjuvants are immunostimulatory substances used to improve and modulate the immune response induced by antigens. A better understanding of the antigen-adjuvant interactions is necessary to develop future effective vaccine. In this study, Taylor dispersion analysis (TDA) was successfully implemented to characterize the interactions between a polymeric adjuvant (poly(acrylic acid), SPA09) and a vaccine antigen in development for the treatment of . TDA allowed one to rapidly determine both (i) the size of the antigen-adjuvant complexes under physiological conditions and (ii) the percentage of free antigen in the adjuvant/antigen mixture at equilibrium and finally get the interaction parameters (stoichiometry and binding constant). The complex sizes obtained by TDA were compared to the results obtained by transmission electron microscopy, and the binding parameters were compared to results previously obtained by frontal analysis continuous capillary electrophoresis.
疫苗佐剂是免疫刺激性物质，用于改善和调节抗原诱导的免疫反应。为了开发未来有效的疫苗，有必要更好地了解抗原-佐剂的相互作用。在这项研究中，成功地实施了泰勒分散分析（TDA）来表征正在开发用于治疗 的疫苗抗原与聚合佐剂（聚丙烯酸，SPA09）之间的相互作用。TDA 允许人们快速确定（i）在生理条件下抗原-佐剂复合物的大小，以及（ii）在平衡时佐剂/抗原混合物中游离抗原的百分比，最终得到相互作用参数（化学计量和结合常数）。通过 TDA 获得的复合物大小与通过透射电子显微镜获得的结果进行了比较，并且将结合参数与以前通过前沿分析连续毛细管电泳获得的结果进行了比较。
