Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha, Hunan, 410078, China; Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong, 518055, China.
Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong, 518055, China; School of Public Health, University of South China, Hengyang, Hunan, 421001, China.
Environ Toxicol Pharmacol. 2021 Aug;86:103658. doi: 10.1016/j.etap.2021.103658. Epub 2021 Apr 16.
Human renal epithelial (HK-2) cells were treated with PM (50 μg/mL) from Shenzhen and Taiyuan, proteomics and bioinformatics were used to screen the differentially expressed proteins (DEPs). A total of 577 DEPs were screened after HK-2 cells exposed to Shenzhen PM, of which 426 were up-regulated and 151 were down-regulated. A total of 1250 DEPs were screened in HK-2 cells after exposure to Taiyuan PM, of which 488 were up-regulated and 185 were down-regulated. The top 10 proteins with the highest number of nodes were screened using the interaction network map of DEPs. HK-2 cells exposed to Shenzhen PM contained CYR61, CTGF, and THBS1 proteins, while HK-2 cells exposed to Taiyuan PM contained ALB, FN1, and CYR61 proteins. Additionally, PM components were detected, PM samples from Shenzhen and Taiyuan induced obvious changes in DEPs expression, the difference in DEPs between the two cities was probably associated with the different PM components.
采用蛋白质组学和生物信息学技术，筛选人肾上皮（HK-2）细胞经深圳和太原 PM（50μg/ml）处理后的差异表达蛋白（DEPs）。深圳 PM 作用 HK-2 细胞后筛选出 577 个 DEPs，其中 426 个上调，151 个下调；太原 PM 作用 HK-2 细胞后筛选出 1250 个 DEPs，其中 488 个上调，185 个下调。采用 DEPs 互作网络图谱筛选出节点数最多的前 10 个蛋白。深圳 PM 作用 HK-2 细胞后含有 CYR61、CTGF 和 THBS1 蛋白，而太原 PM 作用 HK-2 细胞后含有 ALB、FN1 和 CYR61 蛋白。此外，还检测了 PM 成分，深圳和太原的 PM 样品诱导 DEPs 表达明显变化，两城市间 DEPs 的差异可能与不同的 PM 成分有关。
