School of Public Health, University of South China, Hengyang, China.
Shenzhen Center for Disease Control and Prevention, Institute of environment and health, Shenzhen, China.
Toxicol Mech Methods. 2022 Jul;32(6):431-438. doi: 10.1080/15376516.2022.2028328. Epub 2022 Jan 19.
The human bronchial epithelial cells (HBE) and K-ras-silenced HBE cells were treated with fine particulate matter (PM) samples from Taiyuan for 24 h. To screen the proteomic characteristics of PM-induced differentially expressed proteins (DEPs), the Q Exactive mass spectrometer was used. Gene ontology (GO) analysis, Kyoto encyclopedia of genes and genomes (KEGG) analysis, functional prediction, protein-protein interaction (PPI) network analysis, and visualization of differential protein interactions were performed. 251 DEPs in K-ras silenced cells and 535 DEPs in normal HBE cells were identified, respectively. KEGG analysis showed that the differentially expressed proteins of PM-treated cells were related to the biosynthesis of ribosomes, antibiotics, and amino acids. On the other hand, K-ras silenced cells were related to metabolic pathways, RNA transport, and DNA replication. Through the construction of a PPI network, the top 10 hub proteins were screened from the two cell groups, among which MRPL13, RPS20, and EIF1AX were of great significance. Our results indicated that the K-ras gene plays an important role in PM-induced DEPs, and the findings provide a scientific basis for the further study of PM toxic mechanisms and biomarkers.
将人类支气管上皮细胞（HBE）和 K-ras 沉默的 HBE 细胞用来自太原的细颗粒物（PM）样本处理 24 小时。为了筛选 PM 诱导的差异表达蛋白（DEPs）的蛋白质组学特征，使用了 Q Exactive 质谱仪。进行了基因本体论（GO）分析、京都基因与基因组百科全书（KEGG）分析、功能预测、蛋白质-蛋白质相互作用（PPI）网络分析以及差异蛋白相互作用的可视化。分别鉴定了 K-ras 沉默细胞中的 251 个 DEPs 和正常 HBE 细胞中的 535 个 DEPs。KEGG 分析表明，PM 处理细胞的差异表达蛋白与核糖体、抗生素和氨基酸的生物合成有关。另一方面，K-ras 沉默细胞与代谢途径、RNA 转运和 DNA 复制有关。通过构建 PPI 网络，从两个细胞群中筛选出前 10 个枢纽蛋白，其中 MRPL13、RPS20 和 EIF1AX 具有重要意义。我们的结果表明，K-ras 基因在 PM 诱导的 DEPs 中起重要作用，这些发现为进一步研究 PM 毒性机制和生物标志物提供了科学依据。
