Luo Ting, Weng You, Huang Zhuizui, Zhao Yao, Jin Yuanxiang
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2021 Aug;246:109043. doi: 10.1016/j.cbpc.2021.109043. Epub 2021 Apr 16.
Microplastics (MPs) and pesticides are two kinds of ubiquitous pollutants that can pose a health risk to aquatic organisms. However, researches about the combined effects of MPs and pesticides are very limited. A simple combined exposure model was established in this study, adult zebrafish were exposed to 100 μg/L imidacloprid (IMI), 20 μg/L polystyrene microplastics (PS), and a combination of PS and IMI (PS + IMI) for 21 days. The results demonstrated that exposure to PS and IMI inhibited the growth of zebrafish and altered the levels of glycolipid metabolism and oxidative stress-related biochemical parameters. While gene expression analysis revealed that, compared with PS or IMI treatment group, combined exposure caused a greater change in gene expression levels involving the process of glycolipid metabolism (Gk, Hk1, Aco, PPar-α, Cpt1, Acc, Fas, PPar-γ, Apo) and inflammatory response (IL-1β, IL-6, IL-8, TNF-α, IL-10). The results demonstrated that even combined exposure of low concentrations of PS and IMI could cause more severe hepatotoxicity in zebrafish, especially in terms of gene transcription. And more combined toxicity studies are essential for MPs and pesticides risk assessment.
微塑料（MPs）和农药是两种普遍存在的污染物，会对水生生物构成健康风险。然而，关于微塑料和农药联合效应的研究非常有限。本研究建立了一个简单的联合暴露模型，将成年斑马鱼暴露于100μg/L吡虫啉（IMI）、20μg/L聚苯乙烯微塑料（PS）以及PS与IMI的组合（PS + IMI）中21天。结果表明，暴露于PS和IMI会抑制斑马鱼的生长，并改变糖脂代谢水平和氧化应激相关生化参数。而基因表达分析显示，与PS或IMI处理组相比，联合暴露导致涉及糖脂代谢过程（Gk、Hk1、Aco、PPar-α、Cpt1、Acc、Fas、PPar-γ、Apo）和炎症反应（IL-1β、IL-6、IL-8、TNF-α、IL-10）的基因表达水平发生更大变化。结果表明，即使低浓度的PS和IMI联合暴露也会在斑马鱼中引起更严重的肝毒性，尤其是在基因转录方面。更多的联合毒性研究对于微塑料和农药的风险评估至关重要。
