牙龈组织、龈沟液、唾液和血浆中牙周炎相关 miRNA 的分析。

Analysis of periodontitis-associated miRNAs in gingival tissue, gingival crevicular fluid, saliva and blood plasma.

机构信息

Institute of Odontology, Faculty of Medicine, Vilnius University, Vilnius, Lithuania; Vilnius University Hospital Zalgiris Clinic, Vilnius, Lithuania.

出版信息

Arch Oral Biol. 2021 Jun;126:105125. doi: 10.1016/j.archoralbio.2021.105125. Epub 2021 Apr 10.

DOI:10.1016/j.archoralbio.2021.105125

PMID:33862403

Abstract

OBJECTIVE

Periodontitis (PD) is a chronic inflammatory disease which is associated with multiple systemic comorbidities, including rheumatoid arthritis (RA), meanwhile the etiopathology of PD may be modulated by various factors including microRNA (miRNA). The present study aimed to reveal miRNAs associated with PD in gingival tissue, gingival crevicular fluid (GCF), saliva, plasma and to assess the possible influence of RA.

DESIGN

The cross-sectional study included 30 patients with PD and 31 periodontally healthy participants. A total of 25 participants were additionally diagnosed with RA. Microarray analysis of eight gingival tissue samples was performed and four PD-associated miRNAs were selected: miR-199a-5p, miR-483-5p, miR-3198 and miR-4299. Target miRNAs were further assessed by means of RT-qPCR in 61 gingival tissue samples and corresponding bodily fluids - GCF, saliva and plasma.

RESULTS

The upregulation of miR-199a-5p and downregulation of miR-4299 in gingival tissue was associated with the presence of PD and RA (P < 0.05). GCF level of miR-3198 was higher amongst participants with PD (P = 0.019) and showed a good diagnostic ability (AUC = 0.72, P = 0.008). Increased miR-199a-5p salivary level and decreased miR-199a-5p plasma level were observed amongst patients with worse clinical status of PD (P < 0.05). MiR-3198 and miR-4299 combination in GCF demonstrated AUC value of 0.86 and reached sensitivity of 68 % and specificity of 96 %.

CONCLUSIONS

Aberrant expression of miR-199a-5p, miR-483-5p, miR-3198, miR-4299 in gingival tissues is associated with the presence and/or severity of PD. MiR-3198, miR-4299 level in GCF and miR-199a-5p level in plasma strongly correlated with PD, demonstrating significant diagnostic performance.

摘要

目的

牙周炎（PD）是一种慢性炎症性疾病，与多种系统性合并症有关，包括类风湿关节炎（RA），同时 PD 的病因可能受多种因素的调节，包括 microRNA（miRNA）。本研究旨在揭示与牙龈组织、龈沟液（GCF）、唾液、血浆中 PD 相关的 miRNA，并评估 RA 的可能影响。

设计

本横断面研究纳入了 30 名 PD 患者和 31 名牙周健康的参与者。共有 25 名参与者被额外诊断为 RA。对 8 个牙龈组织样本进行微阵列分析，选择了 4 个与 PD 相关的 miRNA：miR-199a-5p、miR-483-5p、miR-3198 和 miR-4299。通过 RT-qPCR 进一步评估了 61 个牙龈组织样本和相应的体液-GCF、唾液和血浆中的靶 miRNA。

结果

牙龈组织中 miR-199a-5p 的上调和 miR-4299 的下调与 PD 和 RA 的存在有关（P<0.05）。PD 患者的 GCF 水平 miR-3198 更高（P=0.019），且具有良好的诊断能力（AUC=0.72，P=0.008）。在 PD 临床状况较差的患者中，观察到 miR-199a-5p 的唾液水平升高和 miR-199a-5p 的血浆水平降低（P<0.05）。GCF 中 miR-3198 和 miR-4299 的组合 AUC 值为 0.86，达到了 68%的敏感性和 96%的特异性。