Institute of Odontology, Faculty of Medicine, Vilnius University.
Institute of Biosciences, Life Sciences Center, Vilnius University.
J Oral Sci. 2022 Jan 19;64(1):11-16. doi: 10.2334/josnusd.21-0282. Epub 2021 Oct 25.
The present study was performed to assess the associations of gingival crevicular fluid (GCF) microRNAs miR-140-3p, miR-145-5p, miR-146a-5p, and miR-195-5p with periodontitis (PD) and to evaluate the possible influence of rheumatoid arthritis (RA) in this context.
GCF samples were collected from 134 individuals with PD and 76 periodontally healthy individuals, with or without RA. After miRNA extraction from GCF, the levels of miR-140-3p, miR-145-5p, miR-146a-5p, and miR-195-5p were assessed using RT-qPCR.
MiR-146a-5p levels were significantly lower among the patients with PD than among the healthy individuals (P < 0.001) and negatively correlated with PD severity based on PD stage and periodontal outcome parameters (P < 0.05). Patients with severe PD had higher GCF levels of miR-140-3p and miR-145-5p than did periodontally healthy individuals (P < 0.05). Significant AUC values for diagnosis of severe PD were revealed for miR-140-3p (AUC = 0.614, P = 0.022), miR-145-5p (AUC = 0.621, P = 0.016) and miR-146a-5p (AUC = 0.702, P < 0.001). Combination of the aforementioned miRNAs increased the diagnostic performance (AUC = 0.709, P < 0.001).
It was demonstrated that miR-140-3p, miR-145-5p and miR-146a-5p were associated with PD and would be potentially effective for GCF-based non-invasive periodontitis diagnostics in patients with and without RA.
本研究旨在评估龈沟液（GCF）microRNAs miR-140-3p、miR-145-5p、miR-146a-5p 和 miR-195-5p 与牙周炎（PD）的相关性，并评估类风湿关节炎（RA）在此背景下的可能影响。
从 134 名 PD 患者和 76 名牙周健康个体（伴或不伴 RA）中采集 GCF 样本。从 GCF 中提取 miRNA 后，使用 RT-qPCR 评估 miR-140-3p、miR-145-5p、miR-146a-5p 和 miR-195-5p 的水平。
与牙周健康个体相比，PD 患者的 miR-146a-5p 水平显著降低（P<0.001），且与 PD 分期和牙周结局参数的 PD 严重程度呈负相关（P<0.05）。与牙周健康个体相比，严重 PD 患者的 GCF 中 miR-140-3p 和 miR-145-5p 水平更高（P<0.05）。miR-140-3p（AUC=0.614，P=0.022）、miR-145-5p（AUC=0.621，P=0.016）和 miR-146a-5p（AUC=0.702，P<0.001）对诊断严重 PD 的 AUC 值有显著意义。上述 miRNA 的组合提高了诊断性能（AUC=0.709，P<0.001）。
研究表明，miR-140-3p、miR-145-5p 和 miR-146a-5p 与 PD 相关，对于伴或不伴 RA 的患者基于 GCF 的非侵入性牙周炎诊断可能具有潜在的有效性。
