牙周炎患者中 miR-30b-3p 和 miR-125b-1-3p 的表达及临床意义。

The expression and clinical significance of miR-30b-3p and miR-125b-1-3p in patients with periodontitis.

机构信息

Department of Stomatology, China Resources and WISCO General Hospital, No. 209, Yejin Avenue, Qingshan District, Wuhan, 430080, Hubei, China.

出版信息

BMC Oral Health. 2022 Aug 5;22(1):325. doi: 10.1186/s12903-022-02360-6.

DOI:10.1186/s12903-022-02360-6

PMID:35931990

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9354350/

Abstract

OBJECTIVE

Periodontitis is a chronic inflammatory infectious disease caused by the deposition of dental plaque on the tooth surface, leading to adverse systemic consequences. Accumulating evidence shows that dysregulated microRNAs (miRNAs) are associated with the disease severity of periodontitis. Herein, we report two novel miRNAs, miR-30b-3p and miR-125b-1-3p, in the context of periodontitis and their relationships with disease severity of periodontitis.

METHODS

The miRNA profiles of gingival crevicular fluid (GCF) samples were used to screen differentially expressed miRNAs (DEmiRNAs) between periodontitis patients and periodontally healthy individuals. Clinical human GCF samples were collected from 80 patients diagnosed with periodontitis (PD +) for the first time and 100 periodontally healthy individuals (PD-). The severity of periodontitis was categorized into mild/moderate (MPD) and severe (SPD) groups. The expressions of miR-30b-3p and miR-125b-1-3p were determined by quantitative real-time PCR. The levels of IL-1β, IL-6, and TNF-α were determined by ELISA methods.

RESULTS

We applied GEO2R bioinformatics tool to analyze the raw data of the GSE89081 dataset and identified miR-30b-3p (|logFC|= 1.987) and miR-125b-1-3p (|logFC|= 1.878) between periodontitis patients and periodontally healthy individuals. It was found that PPD, CAL, BOP, and the relative expression levels of miR-30b-3p and miR-125b-1-3p were all higher in the PD + group than the PD- group, in the SPD group than the MPD group (P < 0.05). The periodontitis patients with high-miR-30b-3p expression exhibited increased PPD, CAL, and BOP compared to those low-miR-30b-3p expression, while high-miR-125b-1-3p expression group showed significant differences on PPD and BOP from low-miR-125b-1-3p expression group (P < 0.05). Pearson correlation analysis demonstrated a significantly positive correlation between the levels of inflammatory cytokines, miR-30b-3p expression, and miR-125b-1-3p expression (P < 0.001). Results of ROC curves showed AUC of 0.878 and 0.927, sensitivity of 0.843 and 0.855, and specificity of 0.791 and 0.801, respectively, when miR-30b-3p and miR-125b-1-3p expression levels were used to diagnose periodontitis.

CONCLUSION

These data unveiled that miR-30b-3p and miR-125b-1-3p expressions may be associated with the pathogenesis of periodontitis.

摘要

目的

牙周炎是一种由牙面菌斑沉积引起的慢性炎症性感染性疾病，可导致不良的全身后果。越来越多的证据表明，失调的 microRNAs（miRNAs）与牙周炎的疾病严重程度有关。在此，我们报告了两种新型 miRNAs，miR-30b-3p 和 miR-125b-1-3p，与牙周炎相关，并探讨了它们与牙周炎疾病严重程度的关系。

方法

利用龈沟液（gingival crevicular fluid，GCF）样本的 miRNA 谱筛选牙周炎患者和牙周健康个体之间差异表达的 miRNAs（DEmiRNAs）。从首次被诊断为牙周炎（PD+）的 80 名患者和 100 名牙周健康个体（PD-）中采集临床人类 GCF 样本。根据牙周炎的严重程度将其分为轻度/中度（MPD）和重度（SPD）组。采用实时定量 PCR 检测 miR-30b-3p 和 miR-125b-1-3p 的表达水平。采用 ELISA 法检测 IL-1β、IL-6 和 TNF-α的水平。

结果

我们应用 GEO2R 生物信息学工具对 GSE89081 数据集的原始数据进行分析，发现 miR-30b-3p（|logFC|=1.987）和 miR-125b-1-3p（|logFC|=1.878）在牙周炎患者和牙周健康个体之间存在差异。结果显示，与 PD-组相比，PD+组的 PPD、CAL、BOP 以及 miR-30b-3p 和 miR-125b-1-3p 的相对表达水平均更高，SPD 组高于 MPD 组（P<0.05）。高 miR-30b-3p 表达的牙周炎患者的 PPD、CAL 和 BOP 均高于低 miR-30b-3p 表达的患者，而高 miR-125b-1-3p 表达组在 PPD 和 BOP 方面与低 miR-125b-1-3p 表达组有显著差异（P<0.05）。Pearson 相关分析表明，炎症细胞因子水平、miR-30b-3p 表达和 miR-125b-1-3p 表达之间呈显著正相关（P<0.001）。ROC 曲线结果显示，miR-30b-3p 和 miR-125b-1-3p 表达水平用于诊断牙周炎的 AUC 分别为 0.878 和 0.927，敏感性分别为 0.843 和 0.855，特异性分别为 0.791 和 0.801。