Howe Rebecca, Evans R L, Ketteridge S W
School of Biological Sciences, Queen Mary and Westfield College, University of London, London E1 4NS, UK.
New Phytol. 1997 Jan;135(1):123-131. doi: 10.1046/j.1469-8137.1997.00622.x.
The presence of copper-binding proteins produced in response to added copper was examined in isolates of Laccaria laccata (Scop, ex Fr.) Cooke and Paxillus involutus (Batsch ex Fr.) Fr. taken from copper-contaminated and uncontaminated sites, and in a single isolate of Scleroderma citrinum Pers. from a contaminated site. Two isolates of Laccaria (GLac4 and ELacl) grew better in 1-5 miu and 2-5 niM copper than a third (Lac3G) and were considered to be more tolerant. Amongst five isolates of P. involutus, three (WJPax2R, GPaxRSp2 and Pax4) were capable of growth in media containing 4-0 mM copper and were regarded as tolerant. All isolates of both Laccaria and Paxillus were capable of some growth in 2-5 mM copper, but S. citrinum was much more copper-sensitive and the concentration had to be reduced at least 10-fold before any growth occurred. Tolerance of isolates was not related to whether they were taken from copper-contaminated or uncontaminated sites. Copper-binding proteins were detected in response to copper in the culture media in the two tolerant isolates of Laccaria (GLac4 and ELacl) but not in the least tolerant isolate. In Paxillus, similar proteins were found in two tolerant isolates (GPaxRSp2 and Pax4) but not in WJPax2R, which was also regarded as tolerant, nor in any of the less tolerant isolates. Copper-binding proteins were not detected in S. citrinum. The copper-binding protein purified from the Laccaria isolate ELacl appeared as a single band in modified SDS-PAGE electrophoresis. Its molecular mass and spectral characteristics were consistent with it being a metallothionein.
对采自铜污染和未污染地点的乳白红菇（Laccaria laccata (Scop, ex Fr.) Cooke）和卷缘桩菇（Paxillus involutus (Batsch ex Fr.) Fr.）的分离菌株，以及采自污染地点的橘黄硬皮马勃（Scleroderma citrinum Pers.）的一个分离菌株，检测了响应添加铜而产生的铜结合蛋白的存在情况。乳白红菇的两个分离菌株（GLac4和ELacl）在1 - 5 μM和2 - 5 mM铜浓度下比第三个菌株（Lac3G）生长得更好，被认为更具耐受性。在卷缘桩菇的五个分离菌株中，三个（WJPax2R、GPaxRSp2和Pax4）能够在含4.0 mM铜的培养基中生长，被视为耐受菌株。乳白红菇和卷缘桩菇的所有分离菌株在2 - 5 mM铜浓度下都能有一定生长，但橘黄硬皮马勃对铜更为敏感，在其出现任何生长之前，铜浓度必须至少降低10倍。分离菌株的耐受性与它们是采自铜污染还是未污染地点无关。在乳白红菇的两个耐受分离菌株（GLac4和ELacl）中检测到了响应培养基中铜而产生的铜结合蛋白，但在耐受性最差的分离菌株中未检测到。在卷缘桩菇中，在两个耐受分离菌株（GPaxRSp2和Pax4）中发现了类似的蛋白，但在同样被视为耐受的WJPax2R中未发现，在任何耐受性较差的分离菌株中也未发现。在橘黄硬皮马勃中未检测到铜结合蛋白。从乳白红菇分离菌株ELacl中纯化的铜结合蛋白在改良的SDS - PAGE电泳中呈现为一条单一带。其分子量和光谱特征与金属硫蛋白一致。
