Department of Otolaryngology, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China.
Department of Otolaryngology, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China.
J Integr Med. 2021 Jul;19(4):354-361. doi: 10.1016/j.joim.2021.01.012. Epub 2021 Jan 21.
Chinese medicine has the potential to modulate allergic rhinitis (AR). There have been studies investigating the treatment efficacy of Yupingfeng San, alone or in combination with other ingredients, in AR, though few have studied the potential mechanisms of these drugs. In the present study, we measured the effects of Jiawei Yupingfeng (JWYPF), a traditional Chinese medicine formula, on mice with ovalbumin-induced AR and explored its underlying mechanism of action.
Forty BALB/c mice were randomly divided into normal control, allergy control and two treatment groups of ten mice each. In the normal control group, mice were sensitized and challenged with saline. The mice in the allergy control and treatment groups were sensitized and challenged with ovalbumin and aluminum hydroxide gel. The treatments of JWYPF and Nasonex were administered intranasally in the AR mice for one week. Several signs of allergic inflammation, such as nasal eosinophils and inflammatory cytokines, were measured to determine the underlying mechanisms.
Mice in the JWYPF and Nasonex groups had significantly lower AR symptom scores than those in the allergy control group (the mean differences between JWYPF and the allergy control, and Nasonex and the allergy control were -2.00 ± 0.35 and -2.40 ± 0.32). After treatment with JWYPF and Nasonex, the levels of ovalbumin-specific IgE and histamine were significantly reduced, as were the levels of interlukin-4 and transforming growth factor-β, while interferon-γ levels were increased (all P < 0.0001, vs. allergy control). These two treatments also significantly inhibited eosinophil and mast cell infiltration into the nasal cavity but were not statistically different from one-another.
JWYPF has a potential therapeutic effect on AR via adjusting the rebalance of T helper 1 and T helper 2.
中药具有调节过敏性鼻炎（AR）的潜力。已经有研究调查了玉屏风散单独或与其他成分联合治疗 AR 的疗效，但很少有研究这些药物的潜在机制。在本研究中，我们测量了加味玉屏风（JWYPF），一种中药配方，对卵清蛋白诱导的 AR 小鼠的影响，并探讨了其潜在的作用机制。
40 只 BALB/c 小鼠随机分为正常对照组、过敏对照组和两个治疗组，每组 10 只。在正常对照组中，小鼠用生理盐水致敏和激发。过敏对照组和治疗组的小鼠用卵清蛋白和氢氧化铝凝胶致敏和激发。AR 小鼠给予 JWYPF 和雷诺考特鼻腔内给药治疗一周。测量几种过敏炎症的迹象，如鼻嗜酸性粒细胞和炎症细胞因子，以确定潜在的机制。
JWYPF 组和雷诺考特组的 AR 症状评分明显低于过敏对照组（JWYPF 组与过敏对照组、雷诺考特组与过敏对照组的平均差异分别为-2.00±0.35 和-2.40±0.32）。用 JWYPF 和雷诺考特治疗后，卵清蛋白特异性 IgE 和组胺水平明显降低，白细胞介素-4 和转化生长因子-β水平降低，干扰素-γ水平升高（均 P<0.0001，与过敏对照组相比）。这两种治疗方法还明显抑制了嗜酸性粒细胞和肥大细胞浸润到鼻腔，但彼此之间没有统计学差异。
JWYPF 通过调节辅助性 T 细胞 1 和辅助性 T 细胞 2 的再平衡，对 AR 具有潜在的治疗作用。
