环状 RNA_0000190 及其靶 microRNA-767-5p 失调，与多发性骨髓瘤患者的风险分层及预后相关。
Circular RNA_0000190 and its target microRNA-767-5p are dysregulated, and they are related to risk stratification as well as prognosis in multiple myeloma patients.
机构信息
Department of Hematology, The First People's Hospital of Jingmen, No. 67 Xiangshan Avenue, Jingmen, 448000, People's Republic of, China.
Department of Cardiovascular Medicine, The First People's Hospital of Jingmen, Jingmen, 448000, China.
出版信息
Ir J Med Sci. 2022 Apr;191(2):671-679. doi: 10.1007/s11845-021-02586-3. Epub 2021 Apr 16.
BACKGROUND
This study aimed to explore the correlation between circular RNA_0000190 (circ_0000190) and microRNA-767-5p (miR-767-5p), and their correlations with biochemical indices, risk stratification, treatment response, and survival in multiple myeloma (MM) patients.
METHODS
Bone marrow (BM) plasma cells of 86 MM patients (during standard diagnostic procedures) and 30 healthy donors (HDs) (examination of the eligibility for BM transplantation) were obtained, among which circ_0000190 and miR-767-5p expressions were detected using reverse transcription quantitative polymerase chain reaction. In MM patients, Durie-Salmon stage and International Staging System (ISS) stage were assessed. Clinical responses (including complete response (CR) and objective response rate (ORR)) were assessed. The progression-free survival (PFS) and overall survival (OS) were calculated.
RESULTS
Circ_0000190 was decreased, but miR-767-5p was increased in MM patients compared with HDs. Circ_0000190 was negatively correlated with miR-767-5p in both HDs and MM patients. In MM patients, circ_0000190 was negatively correlated with ISS stage, serum creatinine, beta-2-microglobulin, and lactate dehydrogenase but was positively correlated with albumin. Whereas an opposite trend in miR-767-5p was observed. Regarding clinical response, circ_0000190 had the value for predicting increased ORR, while miR-767-5p had the value for predicting decreased CR and ORR. Circ_0000190 high expression was correlated with better PFS and OS, while miR-767-5p high expression was correlated with worse PFS and OS. Multivariate Cox's analyses revealed circ_0000190 high expression as an independent factor predicting better OS.
CONCLUSION
Circ_0000190 and its target miR-767-5p are dysregulated, and they are related to risk stratification and prognosis in MM patients.
背景
本研究旨在探讨环状 RNA_0000190（circ_0000190）与微小 RNA-767-5p（miR-767-5p）之间的相关性，及其与多发性骨髓瘤（MM）患者的生化指标、风险分层、治疗反应和生存之间的相关性。
方法
采集 86 例 MM 患者（在标准诊断过程中）和 30 例健康供者（HDs）（骨髓移植资格检查）的骨髓（BM）浆细胞，采用逆转录定量聚合酶链反应检测 circ_0000190 和 miR-767-5p 的表达。在 MM 患者中，评估 Durie-Salmon 分期和国际分期系统（ISS）分期。评估临床反应（包括完全缓解（CR）和客观缓解率（ORR））。计算无进展生存期（PFS）和总生存期（OS）。
结果
与 HDs 相比，MM 患者的 circ_0000190 降低，而 miR-767-5p 升高。circ_0000190 在 HDs 和 MM 患者中均与 miR-767-5p 呈负相关。在 MM 患者中，circ_0000190 与 ISS 分期、血清肌酐、β2-微球蛋白和乳酸脱氢酶呈负相关，与白蛋白呈正相关。而 miR-767-5p 则呈现相反的趋势。在临床反应方面，circ_0000190 有预测增加 ORR 的价值，而 miR-767-5p 有预测降低 CR 和 ORR 的价值。circ_0000190 高表达与更好的 PFS 和 OS 相关，而 miR-767-5p 高表达与更差的 PFS 和 OS 相关。多变量 Cox 分析显示 circ_0000190 高表达是预测 OS 更好的独立因素。
结论
circ_0000190 及其靶 miR-767-5p 失调，与 MM 患者的风险分层和预后相关。
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