Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
College of Aquaculture and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
Mar Biotechnol (NY). 2021 Jun;23(3):389-401. doi: 10.1007/s10126-021-10032-9. Epub 2021 Apr 17.
The black carp (Mylopharyngodon piceus) is an important carnivorous freshwater-cultured species. To understand the molecular basis underlying the response of black carp to fasting, we used RNA-Seq to analyze the liver and brain transcriptome of fasting fish. Annotation to the NCBI database identified 66,609 unigenes, of which 22,841 were classified into the Gene Ontology database and 15,925 were identified in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Comparative analysis of the expression profile between fasting and normal feeding fish revealed 13,737 differentially expressed genes (P < 0.05), of which 12,480 were found in liver tissue and 1257 were found in brain tissue. The KEGG pathway analysis showed significant differences in expression of genes involved in metabolic and immune pathways, such as the insulin signaling pathway, PI3K-Akt signaling pathway, cAMP signaling pathway, FoxO signaling pathway, AMPK signaling pathway, endocytosis, and apoptosis. Quantitative real-time PCR analysis confirmed that expression of the genes encoding the factors involved in those pathways differed between fasting and feeding fish. These results provide valuable information about the molecular response mechanism of black carp under fasting conditions.
黑鲤（Mylopharyngodon piceus）是一种重要的肉食性淡水养殖鱼类。为了了解黑鲤对禁食反应的分子基础，我们使用 RNA-Seq 分析了禁食鱼的肝脏和脑组织转录组。注释到 NCBI 数据库中鉴定出 66609 个基因，其中 22841 个被分类到基因本体数据库中，15925 个在京都基因与基因组百科全书（KEGG）数据库中得到鉴定。禁食和正常摄食鱼之间的表达谱比较分析显示，有 13737 个差异表达基因（P<0.05），其中 12480 个在肝脏组织中发现，1257 个在脑组织中发现。KEGG 途径分析表明，参与代谢和免疫途径的基因表达存在显著差异，如胰岛素信号通路、PI3K-Akt 信号通路、cAMP 信号通路、FoxO 信号通路、AMPK 信号通路、内吞作用和细胞凋亡。实时定量 PCR 分析证实，这些途径中涉及的基因编码因子的表达在禁食和摄食鱼之间存在差异。这些结果为黑鲤在禁食条件下的分子反应机制提供了有价值的信息。
