Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; CCMAR/CIMAR Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
Fish Shellfish Immunol. 2022 Aug;127:366-374. doi: 10.1016/j.fsi.2022.06.031. Epub 2022 Jun 27.
A growing number of studies identified long non-coding RNAs (lncRNAs) to be closely associated with immune function through the regulation of immune cell differentiation and immune cell effector function. Here we tested whether lncRNAs are involved in immune function in black carp (Mylopharyngodon piceus) through the exposure to Aeromonas hydrophila and analysis of the spleen gene expression response using RNA-seq. A total of 9036 lncRNAs were identified with high confidence. Differential expression analysis identified a total of 3558 DElncRNAs (Differential expression lncRNA) involved in A. hydrophila infection and 4526 target genes corresponding to DElncRNAs. After screening 4526 target genes in the InnateDB database, a total of 150 immunity genes were identified. After GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis of the obtained immunity genes, the Toll-like receptor (TLR) signaling pathway, TLR2, TLR3, TLR5, and TLR8 were identified as particularly significant in A. hydrophyla-resistant black carp. At the same time, the Ras signaling pathway was particularly enriched in the spleen of susceptible black carp. Analysis of PPI (protein-protein interaction) networks of the obtained immune genes identified SRC (SRC Proto-Oncogene), MYD88 (Myeloid differentiation primary response 88), MAPK3 (Mitogen-Activated Protein Kinase 3), MYC (MYC Proto-Oncogene) as main hub genes regulated by lncRNA and possibly mediating a mechanism of susceptibility to bacteria. These results establish a functional role of lncRNAs and a mechanistic base for the immune response in black carp resistant to A. hydrophila.
越来越多的研究表明，长链非编码 RNA（lncRNA）通过调节免疫细胞分化和免疫细胞效应功能，与免疫功能密切相关。在这里，我们通过暴露于嗜水气单胞菌并使用 RNA-seq 分析脾脏基因表达反应，测试了 lncRNA 是否参与了黑鲩（Mylopharyngodon piceus）的免疫功能。总共鉴定出了 9036 个具有高可信度的 lncRNA。差异表达分析确定了总共 3558 个参与嗜水气单胞菌感染的 DElncRNA（差异表达 lncRNA）和 4526 个对应于 DElncRNA 的靶基因。在 InnateDB 数据库中筛选 4526 个靶基因后，共鉴定出 150 个免疫基因。对获得的免疫基因进行 GO（基因本体论）和 KEGG（京都基因与基因组百科全书）富集分析后，发现 Toll 样受体（TLR）信号通路、TLR2、TLR3、TLR5 和 TLR8 在抗嗜水气单胞菌黑鲩中特别显著。同时， Ras 信号通路在易感黑鲩的脾脏中特别富集。对获得的免疫基因的 PPI（蛋白质-蛋白质相互作用）网络分析表明，SRC（Src 原癌基因）、MYD88（髓样分化初级反应 88）、MAPK3（丝裂原活化蛋白激酶 3）、MYC（MYC 原癌基因）作为受 lncRNA 调控的主要枢纽基因，可能介导了对细菌易感性的机制。这些结果确立了 lncRNA 在黑鲩抵抗嗜水气单胞菌的免疫反应中的功能作用和机制基础。
