Department of Anesthesiology, University of Vermont Larner College of Medicine, Burlington, VT, 05405, USA; Department of Neurological Sciences, University of Vermont Larner College of Medicine, Burlington, VT, 05405, USA; Department of Pediatrics, University of Vermont Larner College of Medicine, Burlington, VT, 05405, USA.
Department of Neurological Sciences, University of Vermont Larner College of Medicine, Burlington, VT, 05405, USA.
Mech Ageing Dev. 2021 Jun;196:111491. doi: 10.1016/j.mad.2021.111491. Epub 2021 Apr 14.
Preeclampsia, a hypertensive disorder of pregnancy, complicates up to 10 % of all pregnancies and increases the risk for perinatal stroke in offspring. The mechanism of this increase is unknown, but may involve vascular dysfunction. The goal of this study was to evaluate the effect of experimental preeclampsia (ePE) on cerebrovascular function in offspring to eludciate a possible mechanism for this association. Dams were fed a high cholesterol diet beginning on day 7 of gestation to induce experimental preeclampsia. Middle cerebral arteries (MCA) and the Vein of Galen (VoG) were isolated from pups from ePE dams and compared to pups from normal pregnant (NP) dams at postnatal days 16, 23, and 30 and studied pressurized in an arteriograph chamber. Markers of inflammation and oxidative stress were measured in serum. Our results suggest altered structure and function in both MCA and VoG of ePE pups. We also found evidence of systemic inflammation and oxidative stress in ePE pups. These findings provide a potential link between preeclampsia and the occurrence or severity of perinatal stroke.
子痫前期是一种妊娠高血压疾病，影响了多达 10%的妊娠，增加了后代围产期中风的风险。其发病机制尚不清楚，但可能涉及血管功能障碍。本研究旨在评估实验性子痫前期（ePE）对子代脑血管功能的影响，以阐明这种关联的可能机制。从妊娠第 7 天开始，给予母鼠高胆固醇饮食以诱导实验性子痫前期。从 ePE 母鼠的幼崽中分离出大脑中动脉（MCA）和 Galen 静脉（VoG），并与正常妊娠（NP）母鼠的幼崽在出生后第 16、23 和 30 天进行比较，并在加压血管造影室中进行研究。在血清中测量炎症和氧化应激的标志物。我们的结果表明，ePE 幼崽的 MCA 和 VoG 结构和功能发生改变。我们还发现 ePE 幼崽存在全身炎症和氧化应激的证据。这些发现为子痫前期与围产期中风的发生或严重程度之间提供了潜在联系。
