Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, Italy.
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy.
J Chromatogr A. 2021 May 24;1645:462129. doi: 10.1016/j.chroma.2021.462129. Epub 2021 Mar 31.
Comprehensive two-dimensional liquid chromatography is a well-established method for the unraveling of very complex real-world samples. With regard to food and natural products such a technique turned out to be a very promising approach due to its high resolving power and improved identification capability, especially in combination with mass spectrometry. In this context, polyphenols comprise a particular complex class of bioactive compounds, due to their nature and content in commonly consumed foodstuffs, making their analysis challenging. The present contribution shows an overview of the two commonly employed approaches used for polyphenol analysis, viz. RP-LC × RP-LC and HILIC × RP-LC. Furthermore, the latest implementations as well as limitations and future perspectives are critically reported.
二维液相色谱法是一种成熟的方法，用于解析非常复杂的实际样品。对于食品和天然产品，由于其高分辨率和改进的鉴定能力，特别是与质谱联用，该技术已成为一种非常有前途的方法。在这种情况下，多酚类化合物由于其在常见食品中的性质和含量，构成了一类特别复杂的生物活性化合物，这使得它们的分析具有挑战性。本研究概述了用于多酚类化合物分析的两种常用方法，即 RP-LC×RP-LC 和 HILIC×RP-LC。此外，还批判性地报道了最新的应用以及局限性和未来展望。
