Cacciola Francesco, Farnetti Sara, Dugo Paola, Marriott Philip John, Mondello Luigi
Dipartimento di "Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali,", University of Messina, Messina, Italy.
Diabetes Research Institute, Division of Cellular Transplantation of Surgery, University of Miami, Miami, FL, USA.
J Sep Sci. 2017 Jan;40(1):7-24. doi: 10.1002/jssc.201600704. Epub 2016 Sep 16.
Polyphenols are a class of plant secondary metabolites that are recently drawing a special interest because of their broad spectrum of pharmacological effects. As they are characterized by an enormous structural variability, the identification of these molecules in food samples is a difficult task, and sometimes having only a limited number of commercially available reference materials is not of great help. One-dimensional liquid chromatography is the most widely applied analytical approach for their analysis. In particular, the hyphenation of liquid chromatography to mass spectrometry has come to play an influential role by allowing relatively fast tentative identification and accurate quantification of polyphenolic compounds at trace levels in vegetable media. However, when dealing with very complex real-world food samples, a single separation system often does not provide sufficient resolving power for attaining rewarding results. Comprehensive two-dimensional liquid chromatography is a technique of great analytical impact, since it offers much higher peak capacities than separations in a single dimension. In the present review, we describe applications in the field of comprehensive two-dimensional liquid chromatography for polyphenol analysis in real-world food samples. Comprehensive two-dimensional liquid chromatography applications to nonfood matrices fall outside the scope of the current report and will not be discussed.
多酚是一类植物次生代谢产物，因其广泛的药理作用而在最近引起了特别关注。由于它们具有极大的结构变异性，在食品样品中鉴定这些分子是一项艰巨的任务，而且有时仅有数量有限的市售参考物质并无太大帮助。一维液相色谱是其分析中应用最广泛的分析方法。特别是，液相色谱与质谱联用通过能够相对快速地初步鉴定和准确定量蔬菜介质中痕量水平的多酚化合物，发挥了重要作用。然而，在处理非常复杂的实际食品样品时，单一的分离系统往往无法提供足够的分离能力以获得理想的结果。全二维液相色谱是一种具有重大分析影响力的技术，因为它比一维分离提供更高的峰容量。在本综述中，我们描述了全二维液相色谱在实际食品样品中多酚分析领域的应用。全二维液相色谱在非食品基质中的应用不在本报告范围内，将不作讨论。
