Tian Lei, Wang Enze, Lin Xiaolong, Ji Li, Chang Jingjing, Chen Hongping, Wang Jilin, Chen Dazhou, Tran Lam-Son Phan, Tian Chunjie
Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, Jilin, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
BMC Genomics. 2021 Apr 17;22(1):278. doi: 10.1186/s12864-021-07587-1.
Rice, which serves as a staple food for more than half of the world's population, is grown worldwide. The hybridization of wild and cultivated rice has enabled the incorporation of resistance to varying environmental conditions. Endophytic microbiota are known to be transferred with their host plants. Although some studies have reported on the endophytic microbiota of wild and cultivated rice, the inheritance from wild and cultivated rice accessions in next generations, in terms of endophytic microbiota, has not been examined.
In the present study, the endophytic microbial community structures of Asian and African wild and cultivated rice species were compared with those of their F1 offspring. High-throughput sequencing data of bacterial 16S rDNA and fungal internal transcribed spacer regions were used to classify the endophytic microbiota of collected samples of rice. Results indicated that when either African or Asian wild rice species were crossed with cultivated rice accessions, the first generation harbored a greater number of root endophytic fungi than the cultivated parent used to make the crosses. Network analysis of the bacterial and fungal operational taxonomic units revealed that Asian and African wild rice species clustered together and exhibited a greater number of significant correlations between fungal taxa than cultivated rice. The core bacterial genus Acidovorax and the core fungal order Pleosporales, and genera Myrothecium and Bullera connected African and Asian wild rice accessions together, and both the wild rice accessions with their F1 offspring. On the other hand, the core bacterial genus Bradyrhizobium and the core fungal genera Dendroclathra linked the African and Asian cultivated rice accessions together.
This study has theoretical significance for understanding the effect of breeding on the inheritance of endophytic microbiota of rice and identifying beneficial endophytic bacteria and fungi among wild and cultivated rice species, and their F1 offspring.
水稻是世界上一半以上人口的主食，在全球范围内种植。野生稻和栽培稻的杂交使得对不同环境条件的抗性得以整合。已知内生微生物群会与其宿主植物一起转移。尽管一些研究报道了野生稻和栽培稻的内生微生物群，但尚未研究野生稻和栽培稻品种在下一代内生微生物群方面的遗传情况。
在本研究中，将亚洲和非洲野生稻及栽培稻品种的内生微生物群落结构与其F1代后代进行了比较。利用细菌16S rDNA和真菌内转录间隔区的高通量测序数据对采集的水稻样本中的内生微生物群进行分类。结果表明，当非洲或亚洲野生稻品种与栽培稻品种杂交时，第一代的根内生真菌数量比用于杂交的栽培亲本更多。对细菌和真菌操作分类单元的网络分析表明，亚洲和非洲野生稻品种聚集在一起，与栽培稻相比，真菌分类群之间的显著相关性更多。核心细菌属嗜酸菌属和核心真菌目格孢腔菌目以及漆斑菌属和布勒掷孢酵母属将非洲和亚洲野生稻品种以及野生稻品种与其F1代后代连接在一起。另一方面，核心细菌属慢生根瘤菌属和核心真菌属树状枝孢属将非洲和亚洲栽培稻品种连接在一起。
本研究对于理解育种对水稻内生微生物群遗传的影响以及鉴定野生稻和栽培稻品种及其F1代后代中的有益内生细菌和真菌具有理论意义。
