Institute of Microbiology, Department of Biology, ETH Zurich, Zurich, Switzerland.
Laboratory of Inorganic Chemistry, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland.
Methods Enzymol. 2021;650:215-236. doi: 10.1016/bs.mie.2021.01.006. Epub 2021 Feb 18.
Rare-earth elements (REEs) were recently discovered to be biologically significant. The finding was originally made with the methanol dehydrogenase XoxF, which depends on REEs for its activity, and reports of lanthanide-utilizing bacteria have since expanded. Environmental proteomics allows the identification of proteins specifically induced by the presence of lanthanides or can provide insights into the preferred use of lanthanide-dependent and -independent isoenzymes, for example. Here we describe protocols for the growth and subsequent mass spectrometry-based proteome analysis of bacteria obtained from controlled artificial media and from the phyllosphere of the model plant Arabidopsis thaliana. In addition, the use of inductively coupled plasma mass spectrometry (ICP-MS) is described for the quantification of REEs in biological samples.
稀土元素（REEs）最近被发现具有重要的生物学意义。最初的发现是在甲醇脱氢酶 XoxF 中发现的，该酶的活性依赖于 REEs，此后报道了利用镧系元素的细菌。环境蛋白质组学可以鉴定出特定于镧系元素存在而诱导的蛋白质，或者可以深入了解镧系元素依赖和独立同工酶的首选用途。在这里，我们描述了从受控人工培养基和模式植物拟南芥叶围中获得的细菌的生长和随后基于质谱的蛋白质组分析的方案。此外，还描述了使用电感耦合等离子体质谱（ICP-MS）定量生物样品中的 REEs。
