Zhang Huijuan, Zheng Dewei, Yin Longfei, Song Fengming, Jiang Ming
College of Life Science, Taizhou University, Taizhou, China.
National Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Front Plant Sci. 2021 Mar 31;12:652453. doi: 10.3389/fpls.2021.652453. eCollection 2021.
Mediator complex is a multiprotein complex that regulates RNA polymerase II-mediated transcription. Moreover, it functions in several signaling pathways, including those involved in response to biotic and abiotic stresses. We used virus-induced gene silencing (VIGS) to study the functions of two genes, namely and in response to biotic and abiotic stresses in rice. Both genes were differentially induced by (), the causative agent of blast disease, hormone treatment, and abiotic stress. We found that both BMV: OsMED16- and BMV: OsMED25-infiltrated seedlings reduced the resistance to by regulating the accumulation of HO and expression of defense-related genes. Furthermore, BMV: OsMED16-infiltrated seedlings decreased the tolerance to cold by increasing the malondialdehyde (MDA) content and reducing the expression of cold-responsive genes.
中介体复合物是一种多蛋白复合物，可调节RNA聚合酶II介导的转录。此外，它在多种信号通路中发挥作用，包括那些参与对生物和非生物胁迫作出反应的信号通路。我们利用病毒诱导基因沉默（VIGS）来研究水稻中两个基因，即 和 对生物和非生物胁迫的功能。这两个基因都受到稻瘟病病原体（ ）、激素处理和非生物胁迫的差异诱导。我们发现，用BMV: OsMED16和BMV: OsMED25侵染的幼苗通过调节HO的积累和防御相关基因的表达，降低了对 的抗性。此外，用BMV: OsMED16侵染的幼苗通过增加丙二醛（MDA）含量和降低冷响应基因的表达，降低了对寒冷的耐受性。
