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极低频电磁场对胃癌细胞系中miR-21和miR-29上调的影响。

The effect of extremely low frequency electromagnetic fields following on upregulation of miR-21 and miR-29 in gastric cancer cell line.

作者信息

Siasi Elham, Moniri Elaheh

机构信息

Department of Genetic, Collage of Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran.

出版信息

Gastroenterol Hepatol Bed Bench. 2021 Winter;14(1):67-76.

PMID:33868612
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8035540/
Abstract

AIM

Extremely low frequency electromagnetic fields affect miRNAs expression in cancer cell. In this study, electromagnetic fields exposed to low frequency were used to compare miR-21 and miR-29 expressions in a gastric cancer cell line.

BACKGROUND

It has been recently suggested that the low frequency electromagnetic fields probably function as a treatment for cancers.

METHODS

A cultured cell line of gastric cancer was exposed to an electromagnetic radiation system. The cell line was assigned to 4 groups under continuous and discontinuous radiations of 0.25 and 2.5 ml Tesla field strength. Then, the groups were compared with a non-radiation control group. Later, RNA extraction and cDNA synthesis were prepared for miR-21 and miR-29. Real Time PCR method was used to determine how expressions of these two microRNAs differ. Finally, the results were statistically analyzed.

RESULTS

The percentage of cell viability in the electromagnetic field radiation experienced a significant decrease compared to that of the control group. In addition, expression of miRNA-21 and miRNA-29 had a significant increase as the strength of the electromagnetic field radiations was on an upward trend. Similarly, the percentage of cell viability saw a significant decline in the upregulation of miRNA-21 and miRNA-29 regardless of radiation types.

CONCLUSION

Findings of this study showed the therapeutic effect of low frequency electromagnetic fields on the gastric cancer cell line. They also indicated that novel biomarkers (miRNA-21 and miRNA-29) could be proposed as potential treatments of gastric cancer, but the results are required to be well established by future studies.

摘要

目的

极低频电磁场会影响癌细胞中微小RNA（miRNA）的表达。在本研究中，使用暴露于低频的电磁场来比较胃癌细胞系中miR-21和miR-29的表达。

背景

最近有人提出，低频电磁场可能具有治疗癌症的作用。

方法

将培养的胃癌细胞系暴露于电磁辐射系统。该细胞系在0.25和2.5毫特斯拉场强的连续和间断辐射下分为4组。然后，将这些组与非辐射对照组进行比较。随后，制备用于miR-21和miR-29的RNA提取和cDNA合成。采用实时荧光定量PCR方法来确定这两种微小RNA的表达差异。最后，对结果进行统计学分析。

结果

与对照组相比，经历电磁场辐射的细胞活力百分比显著下降。此外，随着电磁场辐射强度呈上升趋势，miRNA-21和miRNA-29的表达显著增加。同样，无论辐射类型如何，miRNA-21和miRNA-29上调时细胞活力百分比均显著下降。

结论

本研究结果显示了低频电磁场对胃癌细胞系的治疗作用。研究结果还表明，可以提出新的生物标志物（miRNA-21和miRNA-29）作为胃癌的潜在治疗方法，但结果需要未来的研究进一步证实。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/98a685123828/GHFBB-14-67-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/0498f2e3766e/GHFBB-14-67-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/a1a1dd810085/GHFBB-14-67-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/da6cc34d7137/GHFBB-14-67-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/9a97281474ce/GHFBB-14-67-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/504bafa49e33/GHFBB-14-67-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/98a685123828/GHFBB-14-67-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/0498f2e3766e/GHFBB-14-67-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/a1a1dd810085/GHFBB-14-67-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/da6cc34d7137/GHFBB-14-67-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/9a97281474ce/GHFBB-14-67-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/504bafa49e33/GHFBB-14-67-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/985d/8035540/98a685123828/GHFBB-14-67-g006.jpg

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