Vige X, Briley M
Department of Biochemical Pharmacology, Centre de Recherche Pierre Fabre, Castres, France.
Neurosci Lett. 1988 Jun 7;88(3):319-24. doi: 10.1016/0304-3940(88)90231-5.
The effect of chronic scopolamine treatment on muscarinic and nicotinic receptors in frontoparietal cortex in rats was investigated. Administration of the muscarinic antagonist, scopolamine (10 mg/kg i.p./day) for 21 days, produced a significant increase in the density of both muscarinic and nicotinic receptors by 27.7% and 12.1% respectively as measured by the specific binding of (-)-[3H]quinuclidinylbenzilate and (-)-[3H]-nicotine. There was no modification in the affinities for these ligands. Rats, bilaterally lesioned with ibotenic acid at the level of nucleus basalis of Meynert, which innervates the frontoparietal cortex, showed no up-regulation of cortical nicotinic receptors after chronic scopolamine treatment, suggesting the importance of the synaptic integrity in the regulation mechanism.
研究了慢性东莨菪碱处理对大鼠额顶叶皮质中M型和N型胆碱能受体的影响。腹腔注射给予M型胆碱能拮抗剂东莨菪碱（10mg/kg/天），持续21天，通过（-）-[3H]喹核醇基苯甲酸酯和（-）-[3H]尼古丁的特异性结合测定，结果显示M型和N型胆碱能受体的密度分别显著增加了27.7%和12.1%。这些配体的亲和力没有改变。用鹅膏蕈氨酸双侧损毁支配额顶叶皮质的Meynert基底核水平的大鼠，在慢性东莨菪碱处理后，皮质N型胆碱能受体没有上调，提示突触完整性在调节机制中的重要性。
