Lyu Jian, Wu Yue, Jin Xin, Tang Zhongqi, Liao Weibiao, Dawuda Mohammed Mujitaba, Hu Linli, Xie Jianming, Yu Jihua, Calderón-Urrea Alejandro
College of Horticulture, Gansu Agricultural University, Lanzhou, China.
College of Agriculture and Forestry Science, Linyi University, Linyi, China.
PeerJ. 2021 Apr 6;9:e10887. doi: 10.7717/peerj.10887. eCollection 2021.
The mechanisms involved in adventitious root formation reflect the adaptability of plants to the environment. Moreover, the rooting process is regulated by endogenous hormone signals. Ethylene, a signaling hormone molecule, has been shown to play an essential role in the process of root development. In the present study, in order to explore the relationship between the ethylene-induced adventitious rooting process and photosynthesis and energy metabolism, the iTRAQ technique and proteomic analysis were employed to ascertain the expression of different proteins that occur during adventitious rooting in cucumber ( L.) seedlings. Out of the 5,014 differentially expressed proteins (DEPs), there were 115 identified DEPs, among which 24 were considered related to adventitious root development. Most of the identified proteins were related to carbon and energy metabolism, photosynthesis, transcription, translation and amino acid metabolism. Subsequently, we focused on S-adenosylmethionine synthase (SAMS) and ATP synthase subunit a (AtpA). Our findings suggest that the key enzyme, SAMS, upstream of ethylene synthesis, is directly involved in adventitious root development in cucumber. Meanwhile, AtpA may be positively correlated with photosynthetic capacity during adventitious root development. Moreover, endogenous ethylene synthesis, photosynthesis, carbon assimilation capacity, and energy material metabolism were enhanced by exogenous ethylene application during adventitious rooting. In conclusion, endogenous ethylene synthesis can be improved by exogenous ethylene additions to stimulate the induction and formation of adventitious roots. Moreover, photosynthesis and starch degradation were enhanced by ethylene treatment to provide more energy and carbon sources for the rooting process.
不定根形成所涉及的机制反映了植物对环境的适应性。此外，生根过程受内源激素信号调控。乙烯作为一种信号激素分子，已被证明在根系发育过程中起重要作用。在本研究中，为了探究乙烯诱导的不定根形成过程与光合作用和能量代谢之间的关系，采用iTRAQ技术和蛋白质组学分析来确定黄瓜（L.）幼苗不定根形成过程中不同蛋白质的表达情况。在5014个差异表达蛋白（DEP）中，鉴定出115个DEP，其中24个被认为与不定根发育相关。大多数鉴定出的蛋白质与碳和能量代谢、光合作用、转录、翻译及氨基酸代谢有关。随后，我们重点关注了S-腺苷甲硫氨酸合成酶（SAMS）和ATP合酶亚基a（AtpA）。我们的研究结果表明，乙烯合成上游的关键酶SAMS直接参与黄瓜不定根的发育。同时，AtpA可能与不定根发育过程中的光合能力呈正相关。此外，在不定根形成过程中，外源施加乙烯可增强内源乙烯合成、光合作用、碳同化能力和能量物质代谢。总之，外源添加乙烯可提高内源乙烯合成，刺激不定根的诱导和形成。此外，乙烯处理可增强光合作用和淀粉降解，为生根过程提供更多能量和碳源。
