Kim Sung-Hyun, Lee DongHan, Lee JinHee, Yang Jun-Young, Seok JiHyun, Jung Kikyung, Lee JongKwon
Division of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Korea.
Toxicol Res. 2021 Jan 1;37(2):277-284. doi: 10.1007/s43188-020-00071-0. eCollection 2021 Apr.
Numerous studies have reported the potential of chemicals for inducing skin sensitization; however, few studies have examined skin sensitization induced by nanomaterials. This study aimed to evaluate skin sensitization induced by metal oxide nanoparticles (NPs) using the ARE-Nrf2 Luciferase KeratinoSens™ assay. Seven different metal oxide NPs, including copper oxide, cobalt oxide, nickel oxide, titanium oxide, cerium oxide, iron oxide, and zinc oxide, were assessed on KeratinoSens™ cells. We selected an appropriate vehicle among three vehicles (DMSO, DW, and culture medium) by assessing the hydrodynamic size at vehicle selection process. Seven metal oxide NPs were analyzed, and their physicochemical properties, including hydrodynamic size, polydispersity, and zeta potential, were determined in the selected vehicle. Thereafter, we assessed the sensitization potential of the NPs using the ARE-Nrf2 Luciferase KeratinoSens™ assay. Copper oxide NPs induced a positive response, whereas cobalt oxide, nickel oxide, titanium oxide, cerium oxide, iron oxide, and zinc oxide NPs induced no response. These results suggest that the ARE-Nrf2 Luciferase KeratinoSens™ assay may be useful for evaluating the potential for skin sensitization induced by metal oxide NPs.
众多研究报告了化学物质诱导皮肤致敏的可能性；然而，很少有研究考察纳米材料诱导的皮肤致敏情况。本研究旨在使用ARE-Nrf2荧光素酶角质形成细胞敏感性检测法（ARE-Nrf2 Luciferase KeratinoSens™ assay）评估金属氧化物纳米颗粒（NPs）诱导的皮肤致敏情况。在KeratinoSens™细胞上评估了七种不同的金属氧化物纳米颗粒，包括氧化铜、氧化钴、氧化镍、氧化钛、氧化铈、氧化铁和氧化锌。在载体选择过程中，通过评估流体动力学尺寸，我们在三种载体（二甲基亚砜、去离子水和培养基）中选择了合适的载体。分析了七种金属氧化物纳米颗粒，并在所选载体中测定了它们的物理化学性质，包括流体动力学尺寸、多分散性和zeta电位。此后，我们使用ARE-Nrf2荧光素酶角质形成细胞敏感性检测法评估了纳米颗粒的致敏潜力。氧化铜纳米颗粒诱导了阳性反应，而氧化钴、氧化镍、氧化钛、氧化铈、氧化铁和氧化锌纳米颗粒未诱导反应。这些结果表明，ARE-Nrf2荧光素酶角质形成细胞敏感性检测法可能有助于评估金属氧化物纳米颗粒诱导皮肤致敏的潜力。
