Dai Shiyu, Wu Qiaoli, Wu Xiaoli, Peng Cheng, Liu Jia, Tang Shuang, Zhang Tao, Deng Fei, Shen Shu
State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
National Biosafety Laboratory, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
Front Cell Infect Microbiol. 2021 Mar 24;11:648077. doi: 10.3389/fcimb.2021.648077. eCollection 2021.
Crimean-Congo hemorrhagic fever (CCHF) is a severe tick-borne viral disease of global concerns due to the increasing incidence and lack of effective treatments. The causative agent, CCHF virus (CCHFV), has been characterized for years; however, its tropism in cell lines of different host and tissue origins remains unclear. This study characterized the susceptibility of 16 human and 6 animal cell lines to CCHFV. Increased viral load and viral nucleoprotein expression, and productive CCHFV replication were detected in human vascular (HUVEC), renal (SW-13 and HEK-293), hepatic (Huh7), and cerebral (U-87 MG) cell lines, which were considered CCHFV-highly permissive cell lines. Renal cell lines derived from monkey and dog could also support CCHFV replication. This study evaluated the susceptibility of different cell lines to CCHFV and identified CCHFV-permissive cell lines. Our findings raise concerns regarding the use of cell lines in studies of CCHFV and may have important implications for further fundamental research, which would promote understanding of CCHFV pathogenesis and transmission, as well as benefit designing strategies for disease prevention and control.
克里米亚-刚果出血热（CCHF）是一种严重的蜱传病毒性疾病，由于发病率不断上升且缺乏有效治疗方法，已成为全球关注的焦点。其病原体克里米亚-刚果出血热病毒（CCHFV）已被研究多年；然而，其在不同宿主和组织来源的细胞系中的嗜性仍不清楚。本研究对16种人类细胞系和6种动物细胞系对CCHFV的易感性进行了表征。在人类血管（HUVEC）、肾脏（SW-13和HEK-293）、肝脏（Huh7）和脑（U-87 MG）细胞系中检测到病毒载量增加、病毒核蛋白表达增加以及CCHFV的有效复制，这些细胞系被认为是对CCHFV高度易感的细胞系。源自猴子和狗的肾细胞系也能支持CCHFV复制。本研究评估了不同细胞系对CCHFV的易感性，并鉴定出对CCHFV易感的细胞系。我们的研究结果引发了对在CCHFV研究中使用细胞系的关注，并可能对进一步的基础研究具有重要意义，这将有助于理解CCHFV的发病机制和传播，以及有利于疾病预防和控制策略的设计。
