从斑马鱼胚胎中分离的侧中胚层细胞整合素介导的细胞黏附分析方案。

Protocol for analysis of integrin-mediated cell adhesion of lateral plate mesoderm cells isolated from zebrafish embryos.

机构信息

Department of Molecular Pathophysiology, Institute of Advanced Medical Sciences, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan.

出版信息

STAR Protoc. 2021 Mar 31;2(2):100428. doi: 10.1016/j.xpro.2021.100428. eCollection 2021 Jun 18.

DOI:10.1016/j.xpro.2021.100428

PMID:33870229

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8044719/

Abstract

Lateral plate mesoderm (LPM) cells differentiate into various cell types including endothelial and hematopoietic cells. In zebrafish embryos, LPM cells migrate toward the midline along the ventral surfaces of somites during which their cell fate specification depends upon efficient integrin-mediated cell adhesion and migration. Herein, we present a protocol for analysis of integrin-mediated cell adhesion of LPM cells isolated from zebrafish embryos. This allows the study of the molecular mechanisms underlying integrin activation required for LPM cell fate specification. For complete details on the use and execution of this protocol, please refer to Rho et al. (2019).

摘要

侧板中胚层 (LPM) 细胞可分化为多种细胞类型，包括内皮细胞和造血细胞。在斑马鱼胚胎中，LPM 细胞沿着体节的腹侧表面向中线迁移，在此过程中，它们的细胞命运决定取决于高效的整合素介导的细胞黏附和迁移。本文提供了一种从斑马鱼胚胎中分离 LPM 细胞以分析整合素介导的细胞黏附的实验方案。这可用于研究 LPM 细胞命运决定所需的整合素激活的分子机制。如需详细了解本实验方案的使用和执行方法，请参考 Rho 等人（2019 年）的文献。