Thümecke Susanne, Schröder Reinhard
Institut für Insektenbiotechnologie, Universität Gießen, Gießen, Germany.
Institut für Biowissenschaften, Universität Rostock, Rostock, Germany.
Dev Dyn. 2022 Sep;251(9):1456-1471. doi: 10.1002/dvdy.347. Epub 2021 May 6.
The evolutionarily conserved odd-skipped related genes odd-skipped (odd), drumstick (drm), sister of odd and bowel (sob), and brother-of-odd-with-entrails-limited (bwl) act downstream of the Notch pathway in various insect tissues including the appendages and the gut. While the function of some of these genes have been analyzed in the adult Tribolium beetle, the expression during and their requirement for embryonic development is not known.
We describe here the embryonic expression patterns of drm, sob, and bwl and analyze the RNAi knockdown phenotypes with emphasize on the appendages and the hindgut. We show that in Tribolium, drm acts independently of other odd-family members in the formation of legs, hindgut, and the dorsal epidermis. Moreover, we establish drm and sob as further markers for segment borders in the appendages that include the gnathobasic mandibles.
We conclude that the regulatory interrelationship among the odd genes differs between Tribolium and Drosophila, where odd and drm seem to act redundantly. In Tribolium, the genes drm and sob uncover the relict of a precoxal joint incorporated in the lateral body wall.
进化上保守的odd-skipped相关基因odd-skipped（odd）、鼓槌（drm）、odd的姐妹基因和肠道基因（sob）以及具有有限内脏的odd兄弟基因（bwl）在包括附肢和肠道在内的各种昆虫组织中，在Notch信号通路的下游发挥作用。虽然其中一些基因在成年赤拟谷盗中的功能已被分析，但它们在胚胎发育期间的表达及其对胚胎发育的需求尚不清楚。
我们在此描述了drm、sob和bwl的胚胎表达模式，并重点分析了附肢和后肠的RNA干扰敲低表型。我们表明，在赤拟谷盗中，drm在腿部、后肠和背侧表皮的形成中独立于其他odd家族成员发挥作用。此外，我们确定drm和sob是附肢（包括颚基上颚）中节边界的进一步标记。
我们得出结论，odd基因之间的调控相互关系在赤拟谷盗和果蝇之间有所不同，在果蝇中odd和drm似乎发挥冗余作用。在赤拟谷盗中，drm和sob基因揭示了并入侧体壁的coxal前关节遗迹。
