Institute of Life Sciences, Nalco Square, Bhubaneswar, Odisha, 751023, India.
School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT) University, Bhubaneswar, Odisha, 751024, India.
Macromol Biosci. 2021 Jun;21(6):e2000381. doi: 10.1002/mabi.202000381. Epub 2021 Apr 19.
Biomimetic matrices offer a great advantage to understand several biological processes including regeneration. The study involves the development of a hybrid biomimetic scaffold and the uniqueness lies in the use of mucin, as a constituent protein. Through this study, the role of the protein in bone regeneration is deciphered through its development as a 3D model. As a first step towards understanding the protein, the interactions of mucin and collagen are determined by in silico studies considering that collagen is the most abundant protein in the bone microenvironment. Both proteins are reported to be involved in bone biology though the exact role of mucin is a topic of investigation. The in silico studies of collagen-mucin suggest to have a proper affinity toward each other, forming a strong basis for 3D scaffold development. The developed 3D scaffold is a double network system comprising of mucin and collagen and vinyl end functionalized polyethylene glycol. In situ deposition of mineral crystals has been performed enzymatically. Biological evaluation of these mineral deposited scaffolds is done in terms of their bone regeneration potential and a comparison of the two systems with and without mineral deposition is presented.
仿生基质在理解包括再生在内的几个生物学过程方面具有很大的优势。本研究涉及一种混合仿生支架的开发，其独特之处在于使用粘蛋白作为组成蛋白。通过这项研究，通过开发 3D 模型来破译蛋白质在骨再生中的作用。作为了解蛋白质的第一步，通过考虑到胶原蛋白是骨微环境中最丰富的蛋白质的计算研究来确定粘蛋白和胶原蛋白的相互作用。这两种蛋白质都被报道参与骨生物学，尽管粘蛋白的确切作用是一个研究课题。胶原蛋白-粘蛋白的计算研究表明它们彼此之间具有适当的亲和力，为 3D 支架的开发奠定了坚实的基础。开发的 3D 支架是一种由粘蛋白和胶原蛋白以及乙烯基末端功能化的聚乙二醇组成的双网络系统。已经通过酶法进行了矿物晶体的原位沉积。对这些矿化支架进行了生物评价，以评估它们的骨再生潜力，并对有和没有矿化沉积的两种系统进行了比较。
