Panda Gyanendra Prasad, Barik Debyashreeta, Dash Mamoni
Institute of Life Sciences, Nalco Square, Bhubaneswar, Odisha 751023, India.
School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT) University, Bhubaneswar, Odisha 751024, India.
ACS Omega. 2024 Mar 25;9(16):17891-17902. doi: 10.1021/acsomega.3c08877. eCollection 2024 Apr 23.
Matrix elasticity helps to direct bone cell differentiation, impact healing processes, and modify extracellular matrix deposition, all of which are required for tissue growth and maintenance. In this work, we evaluated the role of inorganic nanocrystals or mineral inducers such as nanohydroxyapatite, alkaline phosphatase, and nanoclay also known as montmorillonite deposited on vinyl-based hydrogels in generating matrices with different stiffness and their role in cell differentiation. Poly-2-(dimethylamino)ethyl methacrylate (PD) and poly-2-hydroxypropylmethacrylamide (PH) are the two types of vinyl polymers chosen for preparing hydrogels via thermal cross-linking. The hydrogels exhibited porosity, which decreased with an increase in stiffness. Each of the compositions is non-cytotoxic and maintains the viability of pre-osteoblasts (MC3T3-E1) and human bone marrow mesenchymal stem cells (hBMSCs). The PD hydrogels in the presence of ALP showed the highest mineralization ability confirmed through the alizarin assay and a better structural environment for their use as scaffolds for tissue engineering. The study reveals that understanding such interactions can generate hydrogels that can serve as efficient 3D models to study biomineralization.
基质弹性有助于引导骨细胞分化、影响愈合过程以及改变细胞外基质沉积，而这些都是组织生长和维持所必需的。在这项工作中，我们评估了无机纳米晶体或矿物诱导剂（如纳米羟基磷灰石、碱性磷酸酶和也被称为蒙脱石的纳米粘土）沉积在乙烯基水凝胶上在生成具有不同硬度的基质中的作用，以及它们在细胞分化中的作用。聚-2-（二甲氨基）乙基甲基丙烯酸酯（PD）和聚-2-羟丙基甲基丙烯酰胺（PH）是通过热交联制备水凝胶所选用的两种乙烯基聚合物。这些水凝胶具有孔隙率，其随着硬度的增加而降低。每种组合物均无细胞毒性，并能维持前成骨细胞（MC3T3-E1）和人骨髓间充质干细胞（hBMSCs）的活力。在碱性磷酸酶存在下的PD水凝胶通过茜素测定法证实具有最高的矿化能力，并且作为组织工程支架使用时具有更好的结构环境。该研究表明，了解此类相互作用可以生成能够作为研究生物矿化的有效三维模型的水凝胶。
