Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
University of Pittsburgh, Pittsburgh, PA 15260, USA.
Aging (Albany NY). 2021 Apr 19;13(9):12395-12409. doi: 10.18632/aging.202944.
Budding Uninhibited By Benzimidazoles are a group of genes encoding proteins that play central roles in spindle checkpoint during mitosis. Improper mitosis may lead to aneuploidy which is found in many types of tumors. As a key mediator in mitosis, the dysregulated expression of BUBs has been proven to be highly associated with various malignancies, such as leukemia, gastric cancer, breast cancer, and liver cancer. However, bioinformatic analysis has not been applied to explore the role of the BUBs in sarcomas. Herein, we investigate the transcriptional and survival data of BUBs in patients with sarcomas using Oncomine, Gene Expression Profiling Interactive Analysis, Cancer Cell Line Encyclopedia, Kaplan-Meier Plotter, LinkedOmics, and the Database for Annotation, Visualization and Integrated Discovery. We found that the expression levels of BUB1, BUB1B and BUB3 were higher in sarcoma samples and cell lines than in normal controls. Survival analysis revealed that the higher expression levels of BUB1, BUB1B and BUB3 were associated with lower overall and disease-free survival in patients with sarcomas. This study implies that BUB1, BUB1B and BUB3 are potential treatment targets for patients with sarcomas and are new biomarkers for the prognosis of sarcomas.
无抑制素的芽殖是一组编码蛋白质的基因，这些蛋白质在有丝分裂过程中的纺锤体检查点中发挥核心作用。有丝分裂异常可能导致非整倍体，这在许多类型的肿瘤中都有发现。作为有丝分裂过程中的关键介质，BUBs 的表达失调已被证明与各种恶性肿瘤高度相关，如白血病、胃癌、乳腺癌和肝癌。然而，生物信息学分析尚未应用于探索 BUBs 在肉瘤中的作用。在此，我们使用 Oncomine、基因表达谱交互式分析、癌症细胞系百科全书、Kaplan-Meier Plotter、LinkedOmics 和注释、可视化和综合发现数据库，研究了肉瘤患者中 BUBs 的转录和生存数据。我们发现 BUB1、BUB1B 和 BUB3 在肉瘤样本和细胞系中的表达水平高于正常对照。生存分析显示，BUB1、BUB1B 和 BUB3 的高表达水平与肉瘤患者的总生存期和无病生存期较短相关。这项研究表明，BUB1、BUB1B 和 BUB3 可能是肉瘤患者的潜在治疗靶点，也是肉瘤预后的新生物标志物。
