Dantas Lopes Dos Santos Diego, Besegato João Felipe, de Melo Priscila Borges Gobbo, Oshiro Junior João Augusto, Chorilli Marlus, Deng Dongmei, Bagnato Vanderlei Salvador, Rastelli Alessandra Nara de Souza
Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University - UNESP, Araraquara, São Paulo, Brazil.
Department of Restorative Dentistry, School of Dentistry, São Paulo State University - UNESP, Araraquara, São Paulo, Brazil.
Photochem Photobiol. 2021 Sep;97(5):1072-1088. doi: 10.1111/php.13433. Epub 2021 May 17.
Antimicrobial photodynamic therapy (aPDT) is promising for oral decontamination. Curcumin has been used as photosensitizer; however, the hydrophobic properties can negatively affect aPDT. This study evaluated the aPDT efficacy using Cur-loaded Pluronic F-127 micelles against Streptococcus mutans and Candida albicans biofilms. Micelles characterization was performed by zeta potential, dynamic light scattering, transmission electron microscopy, absorption and fluorescence spectroscopy. Cur concentrations, cell viability by CFU mL and confocal microscopy were determined. Data were analyzed by parametric and nonparametric tests under 5%. Cur-loaded Pluronic F-127 exhibited spherical shape, suitable particle size (≤100 nm), adequate polydispersity index, best stability, lower photodegradation and autoaggregation compared to unloaded-Cur. Both microorganisms were sensitive to Cur-loaded Pluronic F-127 micelles aPDT, with minimum inhibitory concentration (MIC) of 270 μm and 2.1093 μm for S. mutans and C. albicans suspended culture, respectively. Cur-loaded Pluronic F-127 aPDT exhibited antibacterial/antifungal effect against the biofilms (~3 log reduction; P ≤ 0.05); however, similar to unloaded (P ≥ 0.05). Confocal images confirmed these results. Cur-loaded Pluronic F-127 micelles exhibited good photo-chemical properties and may be a viable alternative to deliver Cur and to improve aPDT effect during the treatment of dental caries. Moreover, Pluronic micelles can enhance the solubility, stability, permeability and control the release of Cur.
抗菌光动力疗法（aPDT）在口腔去污方面具有广阔前景。姜黄素已被用作光敏剂；然而，其疏水特性可能会对aPDT产生负面影响。本研究评估了负载姜黄素的泊洛沙姆F - 127胶束对变形链球菌和白色念珠菌生物膜的aPDT疗效。通过zeta电位、动态光散射、透射电子显微镜、吸收光谱和荧光光谱对胶束进行表征。测定了姜黄素浓度、通过CFU/mL法测定的细胞活力以及共聚焦显微镜观察结果。数据采用5%以下的参数检验和非参数检验进行分析。与未负载姜黄素相比，负载姜黄素的泊洛沙姆F - 127呈现球形，粒径合适（≤100 nm），多分散指数适宜，稳定性最佳，光降解和自聚集程度更低。两种微生物对负载姜黄素的泊洛沙姆F - 127胶束aPDT均敏感，在悬浮培养中，变形链球菌和白色念珠菌的最低抑菌浓度（MIC）分别为270μm和2.1093μm。负载姜黄素的泊洛沙姆F - 127 aPDT对生物膜表现出抗菌/抗真菌作用（~3个对数级降低；P≤0.05）；然而，与未负载姜黄素的情况相似（P≥0.05）。共聚焦图像证实了这些结果。负载姜黄素的泊洛沙姆F - 127胶束具有良好的光化学性质，在治疗龋齿过程中可能是一种可行的递送姜黄素并改善aPDT效果的替代方法。此外，泊洛沙姆胶束可以提高姜黄素的溶解度、稳定性、渗透性并控制其释放。
