Pabuccu Emre G, Arslanca Tufan, Ucar Ugras, Keles Gurhan, Celikel Ozgul, Pabuccu Recai
Ufuk University School of Medicine, Department of Obstetrics and Gynecology, Ankara, Turkey.
Ufuk University School of Medicine, Department of Obstetrics and Gynecology, Ankara, Turkey.
J Gynecol Obstet Hum Reprod. 2021 Nov;50(9):102150. doi: 10.1016/j.jogoh.2021.102150. Epub 2021 Apr 16.
To compare the efficacy of follicular flushing (FF) with double lumen needle and direct follicular aspiration with single lumen needle in terms of oocyte yield in mono-follicular responder patients undergoing assisted reproduction techniques (ART).
Prospective 'quasi-experimental' study was carried out in an IVF center. Infertile women 18-42 years of age with diminished ovarian reserve who revealed a single follicle >17 mm on the day of oocyte retrieval were included in the study. Follicular flushing up to 8 times was performed in FF group using an 17-G double lumen needle. Direct follicular aspiration using a 17-G single lumen needle without FF was performed in direct aspiration group. Total numbers of collected oocytes, metaphase 2 oocytes, fertilization and pregnancy rates were compared among groups.
A total of 206 women underwent oocyte retrieval procedure; 106 women were assigned to FF and 100 women into direct aspiration arm. Overall oocyte retrieval rate was 50.4% in all cases. The total oocyte retrieval rate was higher (65/106, 61.9%) in FF group, than in direct aspiration group (39/100, 39%, p = 0.001). Metaphase 2 oocyte rate was also higher in FF group (47/106, 44.3% vs 29/100, 29% p = 0.04). Fertilization rates, total number of patients with a cleavage stage embryo and grade 1 cleavage embryo were similar among the groups (p>0.5). Numbers of positive hCG, ongoing pregnancy and miscarriage rates were similar among groups.
Follicular flushing using double lumen needle in mono-follicular responder patients with diminished ovarian reserve yields more oocytes and mature oocytes than direct aspiration of follicles.
比较在接受辅助生殖技术（ART）的单卵泡反应患者中，使用双腔针进行卵泡冲洗（FF）与使用单腔针直接卵泡抽吸在获取卵母细胞方面的效果。
在一家体外受精中心进行前瞻性“准实验性”研究。纳入年龄在18至42岁、卵巢储备功能减退且在取卵当天有一个直径>17mm卵泡的不孕女性。FF组使用17G双腔针进行多达8次的卵泡冲洗。直接抽吸组使用17G单腔针在不进行FF的情况下进行直接卵泡抽吸。比较各组收集的卵母细胞总数、中期2卵母细胞、受精率和妊娠率。
共有206名女性接受了取卵手术；106名女性被分配到FF组，100名女性被分配到直接抽吸组。所有病例的总体取卵率为50.4%。FF组的总取卵率更高（65/106，61.9%），高于直接抽吸组（39/100，39%，p = 0.001）。FF组的中期2卵母细胞率也更高（47/106，44.3%对29/100，29%，p = 0.04）。各组间的受精率、有卵裂期胚胎的患者总数和1级卵裂胚胎相似（p>0.5）。各组间的hCG阳性数、持续妊娠率和流产率相似。
对于卵巢储备功能减退的单卵泡反应患者，使用双腔针进行卵泡冲洗比直接抽吸卵泡能获得更多的卵母细胞和成熟卵母细胞。
