Iotti M, Amicucci A, Stocchi V, Zambonelli A
Dipartimento di Protezione e Valorizzazione Agroalimentare, University of Bologna, Bologna, Italy.
Istituto di Chimica Biologica 'Giorgio Fornaini', Università degli Studi di Urbino, Urbino (PU), Italy.
New Phytol. 2002 Sep;155(3):499-505. doi: 10.1046/j.1469-8137.2002.00486.x.
• Pure cultures of Tuber maculatum , Tuber melanosporum , Tuber aestivum , Tuber macrosporum , Tuber rufum and Tuber brumale were isolated and characterized by morphological and molecular methods. • The Tuber mycelia were isolated from fruit bodies and molecular identification was performed using specific primers, restriction fragment length polymorphism and/or sequence analyses of the ITS region. • The species grew between 1.1 mm wk and 14 mm wk on the selected medium. The mycelium of different Tuber species showed several common morphological features such as hyphal anastomoses, vesicle formation and hyphal aggregation. Differences were found in the frequency of these morphological features and in the hyphal pattern. The isolated mycelia also showed differences in the hyphal branch angle, septal distance, hyphal diameter and rate of growth of the hyphae. • This result opens the possibility of using pure mycelial cultures of Tuber spp. for experimental purposes and for the commercial production of infected truffle plants.
• 分离出了疣孢块菌、黑孢块菌、夏块菌、大孢块菌、红块菌和冬块菌的纯培养物，并通过形态学和分子方法进行了鉴定。
• 从子实体中分离出块菌菌丝体，并使用特异性引物、限制性片段长度多态性和/或ITS区域的序列分析进行分子鉴定。
• 这些物种在选定的培养基上每周生长1.1毫米至14毫米。不同块菌物种的菌丝体表现出几个共同的形态特征，如菌丝吻合、泡囊形成和菌丝聚集。在这些形态特征的频率和菌丝模式上发现了差异。分离出的菌丝体在菌丝分支角度、隔膜距离、菌丝直径和菌丝生长速率上也表现出差异。
• 这一结果为将块菌属的纯菌丝体培养物用于实验目的和商业化生产感染松露的植物开辟了可能性。
