Ng CarL K-Y, Mcainsh Martin R, Gray Julie E, Hunt LeE, Leckie Calum P, Mills Lewis, Hetherington Alistair M
Department of Biological Sciences, Institute of Environmental and Natural Sciences, Lancaster University, Bailrigg, Lancaster, LA1 4YQ, UK.
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2UH, UK.
New Phytol. 2001 Jul;151(1):109-120. doi: 10.1046/j.1469-8137.2001.00152.x.
Calcium is a ubiquitous intracellular signal responsible for controlling numerous cellular processes in both plants and animals. As an example, Ca has been shown to be a second messenger in the signal transduction pathways by which stomatal guard cells respond to external stimuli. Regulated increases in the cytosolic concentration of free calcium ions ([Ca ] ) in guard cells have been observed to be a common intermediate in many of the pathways leading to either opening or closing of the stomatal pore. This observation has prompted investigations into how specificity is encoded in the Ca signal. It has been suggested that the key to generating stimulus-specific calcium signatures lies in the ability to access differentially the cellular machinery controlling calcium influx and release from intracellular stores. Several important components of the calcium-based signalling pathways have been identified in guard cells including cADPR, phospholipase C-InsP , InsP and H O . These data suggest that the pathways for intracellular mobilization of Ca are evolutionarily conserved between plants and animals. ABBREVIATIONS: ABA, abscisic acid; [Ca ] , cytosolic free calcium concentration; [Ca ] , external calcium concentration; I ; inward-rectifying K currents; InsP , inositol-1,4,5-trisphosphate; InsP , inositol hexakisphosphate; PLC, phospholipase C; PLD, phospholipase D; PA, phosphatidic acid; H O , hydrogen peroxide; AAPK, ABA-activated serine-threonine protein kinase; cADPR, cyclic adenosine 5'-diphosphoribose; U73122, 1-(6-{[17â-3-methoxyestra-1,3,5(10)-trien-17-yl]amino}hexyl)-1H-pyrrole-2, 5-dione; RyR; ryanodine receptor; CICR; calcium-induced calcium-release; I , inward calcium current.
钙是一种普遍存在的细胞内信号，负责控制植物和动物体内的众多细胞过程。例如，钙已被证明是气孔保卫细胞响应外部刺激的信号转导途径中的第二信使。在保卫细胞中，已观察到游离钙离子（[Ca]）的胞质浓度的调节性增加是导致气孔孔开放或关闭的许多途径中的常见中间体。这一观察结果促使人们研究钙信号中特异性是如何编码的。有人提出，产生刺激特异性钙信号的关键在于能够以不同方式利用控制钙内流和从细胞内储存库释放的细胞机制。在保卫细胞中已鉴定出基于钙的信号通路的几个重要组成部分，包括环腺苷酸5'-二磷酸核糖（cADPR）、磷脂酶C-肌醇三磷酸（InsP）、肌醇三磷酸（InsP）和过氧化氢（H₂O₂）。这些数据表明，植物和动物之间钙的细胞内动员途径在进化上是保守的。缩写：ABA，脱落酸；[Ca]，胞质游离钙浓度；[Ca]ₒ，外部钙浓度；Iₖ⁺，内向整流钾电流；InsP₃，肌醇-1,4,5-三磷酸；InsP₆，肌醇六磷酸；PLC，磷脂酶C；PLD，磷脂酶D；PA，磷脂酸；H₂O₂，过氧化氢；AAPK，脱落酸激活的丝氨酸-苏氨酸蛋白激酶；cADPR，环腺苷酸5'-二磷酸核糖；U73122，1-(6-{[17β-3-甲氧基雌甾-1,3,5(10)-三烯-17-基]氨基}己基)-1H-吡咯-2,5-二酮；RyR，兰尼碱受体；CICR，钙诱导的钙释放；Iₗₐ，内向钙电流。
