Svensson Mats E, Engström Peter
Evolutionary Biology Centre, Department of Physiological Botany, Uppsala University, Uppsala, Sweden.
Evolutionary Biology Centre, Department of Physiological Botany, Uppsala University, Villavägen 6, S-752 36 Uppsala, Sweden; Current address: Institut für Spezielle Zoologie und Evolutionsbiolog mit Phyletischem Museum, D-07743 Jena, Germany.
New Phytol. 2002 May;154(2):439-450. doi: 10.1046/j.1469-8137.2002.00392.x.
• The relation between the duplication history of the MADS-box gene family of transcription factors and the evolution of plant development is investigated here. The lycopsids, for example the club mosses, constitute the sister group of all other vascular plants, and are therefore interesting from this perspective. • PCR-based methods were used to isolate MADS-box genes from the club moss, Lycopodium annotinum. • In contrast to the previously isolated L. annotinum MADS-box gene LAMB1 (which also contains a so-called K-box), the new L. annotinum genes LAMB2, LAMB4, LAMB6 are structurally similar to most MADS-K-box genes. These genes, and two L. annotinum MADS-box genes, not encoding K-domains, LAMB3 and LAMB5, form a clade distinct from LAMB1. LAMB1 is expressed exclusively in the strobilus unlike LAMB2, LAMB4, LAMB5 and LAMB6, which are expressed in a broad range of organs. • The results imply that the diversification of organ identity MADS-box genes occurred after the split of the lycopsids from the other vascular plants, and that lycopsids have a MADS-box gene family of a type primitive for land plants.
• 本文研究了转录因子MADS-box基因家族的复制历史与植物发育进化之间的关系。例如石松类植物，如石松，构成了所有其他维管植物的姐妹群，因此从这个角度来看很有趣。
• 基于PCR的方法被用于从石松（Lycopodium annotinum）中分离MADS-box基因。
• 与先前分离的石松MADS-box基因LAMB1（其也包含一个所谓的K-box）不同，新的石松基因LAMB2、LAMB4、LAMB6在结构上与大多数MADS-K-box基因相似。这些基因，以及两个不编码K结构域的石松MADS-box基因LAMB3和LAMB5，形成了一个与LAMB1不同的进化枝。与LAMB2、LAMB4、LAMB5和LAMB6不同，LAMB1仅在孢子叶球中表达，而LAMB2、LAMB4、LAMB5和LAMB6在广泛的器官中表达。
• 结果表明，器官特征MADS-box基因的多样化发生在石松类植物与其他维管植物分化之后，并且石松类植物拥有一个对于陆地植物来说是原始类型的MADS-box基因家族。
