Matsui K, Tetsuka T, Nishio T, Hara T
National Agricultural Research Center for Kyushu Okinawa region, Nishigoshi, Kumamoto 861-1192, Japan.
Laboratory of Plant Breeding and Genetics, Tohoku University, Aoba-ku, Sendai 981-8555, Japan.
New Phytol. 2003 Sep;159(3):701-708. doi: 10.1046/j.1469-8137.2003.00840.x.
• Common buckwheat (Fagopyrum esculentum) is a dimorphic self-incompatible plant with either a pin or a thrum flower. It is considered that self-incompatibility, flower morphology, and pollen size are governed by the S supergene. • Here, we produced self-compatible buckwheat lines by an interspecific cross between F. esculentum and F. homotropicum with embryo rescue. • The flower morphology of these lines was long homostyle and pollen size was similar to that of thrum. Pollen size of F plants produced by a cross between a pin plant and the self-compatible plant was similar to that of the self-compatible line and segregated together with flower morphology without exception. The pollen tubes of the self-compatible plants were compatible with styles of the pin plants but incompatible with the styles of thrum plants. However, the pollen tubes of pin flowers were incompatible with the styles of self-compatible plants, but the pollen tubes of thrum flowers were compatible with the styles of self-compatible plants. • These results indicate that the self-compatibility allele, S , retains heteromorphic incompatibility and suggest that the S allele was derived from recombination in the S supergene.
• 普通荞麦（苦荞麦）是一种具有二型性自交不亲和的植物，花型有长花柱花或短花柱花。人们认为自交不亲和性、花形态和花粉大小受S复等位基因控制。
• 在此，我们通过苦荞麦与同型荞麦种间杂交并结合胚拯救技术培育出了自交亲和的荞麦品系。
• 这些品系的花形态为长花柱，花粉大小与短花柱花的相似。长花柱植株与自交亲和植株杂交产生的F植株的花粉大小与自交亲和品系的相似，且毫无例外地与花形态一起分离。自交亲和植株的花粉管与长花柱植株的柱头相容，但与短花柱植株的柱头不相容。然而，长花柱花的花粉管与自交亲和植株的柱头不相容，而短花柱花的花粉管与自交亲和植株的柱头相容。
• 这些结果表明，自交亲和等位基因S保留了异型不亲和性，并表明S等位基因源自S复等位基因的重组。
