Mengoni A, Baker A J M, Bazzicalupo M, Reeves R D, Adigüzel N, Chianni E, Galardi F, Gabbrielli R, Gonnelli C
Dipartimento di Biologia Animale e Genetica, Università di Firenze, via Romana 17-19, I-50125 Firenze, Italy.
School of Botany, The University of Melbourne, Melbourne, VIC 3010, Australia.
New Phytol. 2003 Sep;159(3):691-699. doi: 10.1046/j.1469-8137.2003.00837.x.
• Molecular phylogeny based on ribosomal internal transcribed spacer (ITS) sequences was studied to investigate the phyletic relationships among some nickel (Ni)-hyperaccumulating and nonhyperaccumulating species of the genus Alyssum in relation to their geographic distribution and Ni-hyperaccumulating phenotype. • Thirty-seven samples belonging to 32 taxa were analysed by sequencing the polymerase chain reaction-amplified ITS region and performing neighbor joining, maximum parsimony and maximum likelihood phylogenetic analyses. • The ITS region in the sampled species varied from 221 to 307 bp of ITS1 and from 194 to 251 bp of ITS2. A total of 765 characters was used to infer the phylogeny and the average nucleotide variation detected was 15.15%. • Nickel-hyperaccumulation could have been lost or acquired independently more than once during the speciation of the genus. The geographical location of species could not be related to phylogenetic affinities.
• 基于核糖体内部转录间隔区（ITS）序列进行分子系统发育研究，以探究庭荠属一些镍（Ni）超积累和非超积累物种之间的系统发育关系，及其地理分布与镍超积累表型的关系。
• 通过对聚合酶链反应扩增的ITS区域进行测序，并进行邻接法、最大简约法和最大似然法系统发育分析，对属于32个分类单元的37个样本进行了分析。
• 所采样物种的ITS区域中，ITS1长度在221至307 bp之间，ITS2长度在194至251 bp之间。共使用765个字符来推断系统发育，检测到的平均核苷酸变异为15.15%。
• 在该属物种形成过程中，镍超积累特性可能不止一次地独立丧失或获得。物种的地理位置与系统发育亲缘关系无关。
