Department of Botany, La Trobe University Bundoora, VIC, Australia.
Front Plant Sci. 2013 Aug 20;4:319. doi: 10.3389/fpls.2013.00319. eCollection 2013.
Microbeam studies over the past decade have garnered unique insight into manganese (Mn) homeostasis in plant species that hyperaccumulate this essential mineral micronutrient. Electron- and/or proton-probe methodologies employed to examine tissue elemental distributions have proven highly effective in illuminating excess foliar Mn disposal strategies, some apparently unique to Mn hyperaccumulating plants. When applied to samples prepared with minimal artefacts, these are powerful tools for extracting true 'snapshot' data of living systems. For a range of reasons, Mn hyperaccumulation is particularly suited to in vivo interrogation by this approach. Whilst microbeam investigation of metallophytes is well documented, certain methods originally intended for non-biological samples are now widely applied in biology. This review examines current knowledge about Mn hyperaccumulators with reference to microbeam methodologies, and discusses implications for future research into metal transporters.
过去十年的微束研究为超积累这种必需的矿物微量元素的植物物种中的锰 (Mn) 稳态提供了独特的见解。用于检查组织元素分布的电子和/或质子探针方法已被证明在揭示过量叶 Mn 处置策略方面非常有效，其中一些策略显然是 Mn 超积累植物所特有的。当应用于最小化人为假象的样本时，这些工具对于提取真实的“快照”数据的活体系统是非常有效的。出于多种原因，Mn 超积累特别适合通过这种方法进行体内研究。虽然金属植物的微束研究已有详细记录，但某些最初用于非生物样本的方法现在已广泛应用于生物学领域。本综述参考微束方法检查了关于 Mn 超积累植物的现有知识，并讨论了对未来金属转运体研究的影响。
