Peter Martina, Courty Pierre-Emmanuel, Kohler Annegret, Delaruelle Christine, Martin David, Tagu Denis, Frey-Klett Pascale, Duplessis Sébastien, Chalot Michel, Podila Gopi, Martin Francis
Unité Mixte de Recherche INRA-UHP 1136 'Interactions Arbres/Microorganismes', Centre de Recherches de Nancy, 54280 Champenoux, France.
These authors contributed equally to this work.
New Phytol. 2003 Jul;159(1):117-129. doi: 10.1046/j.1469-8137.2003.00796.x.
• In an effort to discover genes that are expressed in the ectomycorrhizal basidiomycetes Laccaria bicolor and Pisolithus microcarpus, and in P. microcarpus/Eucalyptus globulus ectomycorrhizas, we have sequenced 1519 and 1681 expressed sequence tags (ESTs) from L. bicolor and P. microcarpus cDNA libraries. • Contig analysis resulted in 905 and 806 tentative consensus sequences (unique transcripts) in L. bicolor and P. microcarpus, respectively. For 36% of the ESTs, significant similarities to sequences in databases were detected. The most abundant transcripts showed no similarity to previously identified genes. Sequence redundancy analysis between different developmental stages indicated that several genes were differentially expressed in free-living mycelium and symbiotic tissues of P. microcarpus. • Based on sequence similarity, 11% of L. bicolor unique transcripts were also detected in P. microcarpus. Similarly, L. bicolor and P. microcarpus shared only a low proportion of common transcripts with other basidiomycetous fungi, such as Pleurotus ostreatus and Agaricus bisporus. Such a low proportion of shared transcripts between basidiomycetes suggests, on the one hand, that the variability of expressed transcripts in different fungi and fungal tissues is considerably high. On the other hand, it might reflect the low number of GenBank entries of basidiomycetous origin and stresses the necessity of an additional sequencing effort. • The present ESTs provide a valuable resource for future research on the development and functioning of ectomycorrhizas.
• 为了发现在外生菌根担子菌双色蜡蘑和微小皮伞，以及微小皮伞/蓝桉外生菌根中表达的基因，我们对来自双色蜡蘑和微小皮伞cDNA文库的1519个和1681个表达序列标签（EST）进行了测序。• 重叠群分析分别在双色蜡蘑和微小皮伞中产生了905个和806个初步一致性序列（独特转录本）。对于36%的EST，检测到与数据库中的序列有显著相似性。最丰富的转录本与先前鉴定的基因没有相似性。不同发育阶段之间的序列冗余分析表明，微小皮伞的几个基因在自由生活的菌丝体和共生组织中差异表达。• 根据序列相似性，在微小皮伞中也检测到11%的双色蜡蘑独特转录本。同样，双色蜡蘑和微小皮伞与其他担子菌，如糙皮侧耳和双孢蘑菇，仅共享低比例的共同转录本。担子菌之间如此低比例的共享转录本一方面表明不同真菌和真菌组织中表达转录本的变异性相当高。另一方面，这可能反映了担子菌来源的GenBank条目数量较少，并强调了额外测序工作的必要性。• 目前的EST为未来外生菌根发育和功能的研究提供了宝贵的资源。
