Osaki Hideki, Nomura Kinya, Iwanami Toru, Kanematsu Satoko, Okabe Ikuko, Matsumoto Naoyuki, Sasaki Atsuko, Ohtsu Yoshihiro
National Institute of Fruit Tree Science, Fujimoto, Tsukuba, Japan.
Virus Genes. 2002 Oct;25(2):139-45. doi: 10.1023/a:1020105701017.
Three double-stranded (ds) RNA species (ca. 1.30, 1.27 and 1.23 x 106) were isolated by CF-11 cellulose chromatography from a strain of the violet root rot fungus Helicobasidium mompa recovered from apple roots. Purified virion preparations contained isometric particles about 25 nm in diameter, and also the same three species of dsRNA isolated from total extracts by CF-11 cellulose chromatography. The molecular mass of the coat protein was about 67 K when estimated by SDS-PAGE. The largest dsRNA (referred to as dsRNA1) contains a single, long open reading frame of 1794 nucleotides that encodes a putative polypeptide containing 598 amino acid residues with a molecular mass of 69.9 K. This polypeptide contains amino acid sequence motifs conserved in putative RNA-dependent RNA polymerases of RNA viruses. Phylogenetic analysis revealed similarities to RNA-dependent RNA polymerases from Atkinsonella hypoxylon 2H virus, a member of the family Partitiviridae.
通过CF - 11纤维素柱层析从苹果根中分离出的紫纹羽病菌（Helicobasidium mompa）菌株中，分离出三种双链（ds）RNA（约1.30、1.27和1.23×10⁶）。纯化的病毒粒子制剂含有直径约25nm的等轴颗粒，并且还含有通过CF - 11纤维素柱层析从总提取物中分离出的相同三种dsRNA。通过SDS - PAGE估计，外壳蛋白的分子量约为67K。最大的dsRNA（称为dsRNA1）包含一个1794个核苷酸的单一长开放阅读框，编码一个推定的多肽，该多肽含有598个氨基酸残基，分子量为69.9K。该多肽含有在RNA病毒的推定RNA依赖性RNA聚合酶中保守的氨基酸序列基序。系统发育分析显示与来自Atkinsonella hypoxylon 2H病毒（双分病毒科的成员）的RNA依赖性RNA聚合酶相似。
