Wallin G, Ottosson S, Selldén G
Department of Plant Physiology, University of Göteborg, Carl Skottsbergs gata 22, S-413 19 Göteborg, Sweden.
Swedish Environmental Research Institute (IVL), Box 47086, S-402 58 Göteborg, Sweden.
New Phytol. 1992 Jul;121(3):395-401. doi: 10.1111/j.1469-8137.1992.tb02939.x.
Rates of photosynthesis at different concentrations of CO (0-1850 μmol mol ) were measured using one clone of Norway spruce, Picea abies (L.) Karst. exposed to three different concentrations of ozone for four seasons (1985-8) in open-top chambers. The treatments were non-filtered air+ozone (NF+), non-filtered air (NF) and charcoal-filtered air (CF). The measurements were made on shoots of four different age classes. No significant effects were found on photosynthesis or on leaf conductance to CO in current year shoots. In 1- to 3-yr-old shoots, leaf conductance to CO and rates of net photosynthesis at both 330 μmol mol CO (P 330), and saturating concentrations of CO (P max), decreased with increasing shoot age and ozone concentration, although this was only significant for P 330 in 3-yr-old shoots. In current year shoots the intercellular concentration of CO was significantly higher in NF+-treated shoots compared with CF- and NF-treated shoots, while in the 2- and 3-yr-old shoots it was significantly higher in NF- and NF +-treated shoots compared with CF-treated shoots. The carboxylation efficiency significantly decreased in 2- and 3-yr-old shoots from the NF and NF+ treatments compared with shoots from the CF treatment. The gas phase limitation of photosynthesis decreased with increasing shoot age and ozone concentration.
使用一株挪威云杉（Picea abies (L.) Karst.），在开顶式气室中，于四个季节（1985 - 1988年）暴露于三种不同浓度的臭氧下，测量了不同CO浓度（0 - 1850 μmol mol⁻¹）时的光合速率。处理方式为未过滤空气 + 臭氧（NF +）、未过滤空气（NF）和活性炭过滤空气（CF）。测量在四个不同年龄级别的嫩枝上进行。对于当年生嫩枝，未发现对光合作用或叶片对CO的导度有显著影响。在1至3年生嫩枝中，叶片对CO的导度以及在330 μmol mol⁻¹ CO（P₃₃₀）和饱和CO浓度（Pmax）下的净光合速率，均随着嫩枝年龄和臭氧浓度的增加而降低，不过这仅在3年生嫩枝的P₃₃₀上显著。在当年生嫩枝中，与CF处理和NF处理的嫩枝相比，NF +处理的嫩枝中细胞间CO浓度显著更高，而在2年生和3年生嫩枝中，与CF处理的嫩枝相比，NF处理和NF +处理的嫩枝中细胞间CO浓度显著更高。与CF处理的嫩枝相比，NF和NF +处理的2年生和3年生嫩枝的羧化效率显著降低。光合作用的气相限制随着嫩枝年龄和臭氧浓度的增加而降低。
