Mikkelsen T N, Ro-Poulsen H
Department of Plant Ecology, Botanical Institute, University of Copenhagen, Øuter Farimagsgade 2D, 1353 Copenhagen, Denmark.
New Phytol. 1994 Sep;128(1):153-163. doi: 10.1111/j.1469-8137.1994.tb03998.x.
Physiological effects of ozone exposure over three consecutive growing seasons on current year needles of Norway spruce were studied in open-top chambers, during daily Fumigation cycles in the summer, and after the termination of ozone fumigation in autumn 1990. The trees were exposed to two levels of ozone: charcoal filtered air and non-filtered air to which 30 nl I of ozone was added in three consecutive years from 1988 to 1990, daily from May to September (8 hours a day). Photosynthesis, stomatal conductance, transpiration and chlorophyll fluorescence were studied on selected days. Significant decreases in net photosynthesis and chlorophyll fluorescence (F /F ) were found during periods with co-occurrence of high ozone concentrations And high light intensities, indicating interactions between effects of ozone and photoinhibition. After termination of fumigation enhanced rates of photosynthesis were seen in the trees which had been exposed to ozone. A significant decrease in F /F was found for twigs from ozone treated trees when exposed to severe desiccation.
在开放式气室中，研究了连续三个生长季节臭氧暴露对挪威云杉当年针叶的生理影响，实验于夏季每日熏蒸周期以及1990年秋季臭氧熏蒸结束后进行。树木暴露于两种臭氧水平下：经过活性炭过滤的空气和未过滤的空气，从1988年至1990年连续三年每天从五月至九月（每天8小时）向未过滤空气中添加30 nl/L的臭氧。在选定的日子研究了光合作用、气孔导度、蒸腾作用和叶绿素荧光。在高臭氧浓度和高光强度同时出现的时期，发现净光合作用和叶绿素荧光（Fv/Fm）显著下降，表明臭氧效应与光抑制之间存在相互作用。熏蒸结束后，曾暴露于臭氧的树木光合作用速率有所提高。当暴露于严重干燥环境时，发现经过臭氧处理的树木嫩枝的Fv/Fm显著下降。
