Löser C, Fölsch U R, Mustroph D, Cantor P, Wunderlich U, Creutzfeldt W
Department of Medicine, Georg-August-University of Göttingen, F.R.G.
Pancreas. 1988;3(3):285-91. doi: 10.1097/00006676-198805000-00008.
We investigated the trophic effect on the pancreas of male Wistar rats fed up to 20 days with either raw soybean flour (RSF) containing an active trypsin inhibitor or heat-inactivated soybean flour (HSF). The concentrations of the polyamines putrescine, spermidine, and spermine in the pancreas as well as cholecystokinin (CCK) concentrations in arterial and portal vein plasma were measured. Plasma CCK concentrations were measured by a sensitive radioimmunoassay specific for the sulfated region of CCK, whereas polyamine concentrations are determined by reversed phase high-performance liquid chromatography. The levels of CCK in both arterial and portal vein plasma were significantly higher in RSF- compared with HSF-fed rats, the concentration in the portal vein being twice as high compared with the aorta. A significant increase in pancreatic weight and protein content was positively correlated to an increase in putrescine and spermidine in the pancreas of RSF-fed rats compared with HSF-fed controls, whereas the spermine content did not differ between the two groups. The pancreatic DNA content in RSF-fed rats was significantly above control values of day 20 only. These data support the hypothesis that the trophic effect of soybean trypsin inhibitor on the pancreas is mediated by CCK and that polyamines might play an important role in CCK-induced pancreatic growth.
我们研究了用含有活性胰蛋白酶抑制剂的生大豆粉（RSF）或热灭活大豆粉（HSF）喂养雄性Wistar大鼠长达20天对胰腺的营养作用。测量了胰腺中多胺腐胺、亚精胺和精胺的浓度以及动脉和门静脉血浆中胆囊收缩素（CCK）的浓度。血浆CCK浓度通过对CCK硫酸化区域特异的灵敏放射免疫测定法测量，而多胺浓度通过反相高效液相色谱法测定。与HSF喂养的大鼠相比，RSF喂养的大鼠动脉和门静脉血浆中的CCK水平显著更高，门静脉中的浓度与主动脉相比高出两倍。与HSF喂养的对照组相比，RSF喂养的大鼠胰腺重量和蛋白质含量显著增加，且与胰腺中腐胺和亚精胺的增加呈正相关，而两组之间的精胺含量没有差异。RSF喂养的大鼠胰腺DNA含量仅在第20天显著高于对照值。这些数据支持以下假设：大豆胰蛋白酶抑制剂对胰腺的营养作用由CCK介导，并且多胺可能在CCK诱导的胰腺生长中起重要作用。
