Carr G R, Hinkley M A, LE Tacon F, Hepper C M, Jones M G K, Thomas E
Rothamsted Experimental Station, Harpenden, Hertfordshire, AL5 2JQ, UK.
New Phytol. 1985 Nov;101(3):417-426. doi: 10.1111/j.1469-8137.1985.tb02848.x.
Conditions required for the combined culture of either Glomus caledonium (Nicol. &Gerd.) Trappe &Gerdemann or Glomus mosseae (Nicol. &Gerd.) Gerdemann &Trappe with suspension-cultured plant cells have been investigated. Sucrose levels (0.05 to 0.5%, w/v) lower than those used for growth of plant cells were optimal for hyphal growth of both G. caledonium and G. mosseae. In vitro hyphal growth from chlamydospores of G. caledonium was stimulated by addition of cells of wheat (Triticum aestivum L. cv. Maris Butler), lucerne (Medicago sativa L. cv. Europ) and potato (Solanum tuberosum L. cv. Maris Piper). The presence of wheat cells similarly stimulated hyphal growth from chlamydospores of G. mosseae. Further tests on the effect of lucerne cells on G. caledonium indicated that a volatile substance was involved in the improvement of hyphal growth.
对胶膜菌（Glomus caledonium (Nicol. & Gerd.) Trappe & Gerdemann）或摩西球囊霉（Glomus mosseae (Nicol. & Gerd.) Gerdemann & Trappe）与悬浮培养的植物细胞进行联合培养所需的条件已进行了研究。蔗糖水平（0.05%至0.5%，w/v）低于用于植物细胞生长的水平时，对胶膜菌和摩西球囊霉的菌丝生长最为适宜。添加小麦（Triticum aestivum L. cv. Maris Butler）、苜蓿（Medicago sativa L. cv. Europ）和马铃薯（Solanum tuberosum L. cv. Maris Piper）的细胞可刺激胶膜菌厚垣孢子的体外菌丝生长。小麦细胞的存在同样刺激了摩西球囊霉厚垣孢子的菌丝生长。对苜蓿细胞对胶膜菌影响的进一步测试表明，一种挥发性物质参与了菌丝生长的改善过程。
