Lemoine M C, Gollotte A, Gianinazzi-Pearson V
Laboratoire de Phytoparasitologie, INRA-CNRS, Station de Génétique et d'Amélioration des Plantes, INRA, BV 1540, 21034 Dijon, Cedex, France.
New Phytol. 1995 Jan;129(1):97-105. doi: 10.1111/j.1469-8137.1995.tb03013.x.
Previous studies showed that cell walls of endomycorrhizal fungi belonging to the Acaulosporaceae and Glomaceae contain β (1-3) glucan polymers as well as chitin. Indirect immunolabelling with monoclonal and polyclonal antibodies has been used to investigate the distribution of these structural polysaccharides in cell walls of Glomus mosseae (Nicol. & Gerd.) Gerd. & Trappe and Acaulospora laevis Gerd. & Trappe as they interact with pea and tobacco roots, respectively. The (l-3) glucans were detected in the walls of external hyphae, and of hyphal coils and intercellular hyphae developing in outer root tissues. The glucan component was alkali-insoluble but treatment with chitinase resulted in solubilization of most of the β(1-3) glucans from the fungal wall. A decrease in immunolabelling was associated with thinning out of the hyphal wall as the fungi colonized deeper in the host root, and β(1-3) glucans could not be detected in walls of intercellular hyphae or arbuscules in the parenchyma cortical tissue. The molecular configuration of cell walls of G. mosseae and A. laevis is discussed in relation to fungal morphogenesis and the symbiotic state in endomycorrhiza.
先前的研究表明，属于无梗囊霉科和球囊霉科的丛枝菌根真菌的细胞壁含有β(1-3)葡聚糖聚合物以及几丁质。已使用单克隆抗体和多克隆抗体进行间接免疫标记，以研究这些结构多糖在摩西球囊霉（Nicol. & Gerd.）Gerd. & Trappe和光壁无梗囊霉Gerd. & Trappe细胞壁中的分布，因为它们分别与豌豆和烟草根系相互作用。在外部菌丝、菌丝圈以及在根外部组织中发育的细胞间菌丝的壁中检测到了(1-3)葡聚糖。葡聚糖成分不溶于碱，但用几丁质酶处理会导致大部分β(1-3)葡聚糖从真菌壁中溶解出来。随着真菌在宿主根中定殖更深，免疫标记的减少与菌丝壁变薄相关，并且在薄壁组织皮层组织的细胞间菌丝或丛枝的壁中未检测到β(1-3)葡聚糖。本文结合真菌形态发生和丛枝菌根中的共生状态，讨论了摩西球囊霉和光壁无梗囊霉细胞壁的分子构型。
