Pell E J, Winner W E, Vinten-Johansen C, Mooney H A
Department of Plant Pathology and Environmental Resources Research Institute, The Pennsylvania State University, 211 Buckhout Laboratory, University Park, PA 16802 USA.
Department of General Sciences, Oregon State University, Corvallis, OR 97331 USA.
New Phytol. 1990 Jul;115(3):439-446. doi: 10.1111/j.1469-8137.1990.tb00469.x.
Experiments were conducted to determine the impact of nitrogen and ozone (O ) stress on the growth of domestic radish Raphanus sativus L. cv. Cherry Belle. Plants were grown in field chambers with sub-, optimal and supra-optimal levels of nitrogenous fertilizer. Chamber air was either charcoal-filtered, or supplemented with one of two levels of O . The highest O treatment resulted in significant reduction in weight of hypocotyls and roots while elevated nitrogen treatments resulted in increased weight of all plant parts. Ozone did not affect the weight of plant foliage at any nitrogen level. Plants grown with lower levels of nitrogen had less leaf biomass but the tissue accounted for a greater percentage total weight than did the foliage of higher nitrogen treatments. Relative growth rate of whole plants was not affected by O or nitrogen treatments reflecting compensation in response to both stresses. Ozone-induced depression in biomass was observed in O -treated plants grown with higher nitrogen supply but not in those grown with limiting nitrogen. This observation could reflect compensation at the lower levels of nitrogen supply or inability to detect changes in biomass due to reduced weights of plants grown at the lowest nitrogen supply. The dry weight ratio of sink organs (hypocotyl plus root)/shoot was significantly correlated with the total non-structural carbohydrate (TNC) content of these organs, regardless of treatment. Initially, O induced a significant decrease and nitrogen an increase in percent TNC of sink organs. At later sampling times, plants adjusted to stress as effects on percent TNC were no longer evident.
进行了实验以确定氮和臭氧（O₃）胁迫对国产樱桃萝卜（Raphanus sativus L. cv. Cherry Belle）生长的影响。植株在田间试验箱中生长，施用亚最佳、最佳和超最佳水平的氮肥。试验箱中的空气要么经过活性炭过滤，要么补充两种水平之一的O₃。最高O₃处理导致下胚轴和根的重量显著降低，而氮肥水平升高导致所有植株部分的重量增加。在任何氮水平下，臭氧都不影响植株叶片的重量。施用较低水平氮肥的植株叶片生物量较少，但该组织占总重量的百分比高于施用较高氮肥处理的叶片。整株植物的相对生长速率不受O₃或氮处理的影响，这反映了对两种胁迫的补偿作用。在施用较高氮供应的O₃处理植株中观察到臭氧诱导的生物量下降，但在施用限量氮的植株中未观察到。这一观察结果可能反映了在较低氮供应水平下的补偿作用，或者由于在最低氮供应水平下生长的植株重量减轻而无法检测到生物量的变化。无论处理如何，库器官（下胚轴加根）/地上部分的干重比与这些器官的总非结构性碳水化合物（TNC）含量显著相关。最初，O₃导致库器官TNC百分比显著下降，而氮导致其增加。在后期采样时，植株适应了胁迫，因为对TNC百分比的影响不再明显。
