Department of Chemistry, Payame Noor University of Mashhad (PNU), PO Box 19395-4697, Mashhad, Iran.
Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, 13131-99137, Iran.
J Nanosci Nanotechnol. 2021 Oct 1;21(10):5099-5106. doi: 10.1166/jnn.2021.19367.
The present study aims to prepare and And Characterization of zein nanofibers with simvastatin-loaded in it by electrospinning method using a polymeric matrix prepared from zein solution containing simvastatin antibiotics. The physical, cellular properties and drug release kinetics of the fibers were studied. Fiber formation was investigated by implementing a scanning electron microscope (SEM). The SEM images show a uniform network bead-free fiber structure created from the 1.5% simvastatin/zeinsolution. The viscosity of the 1.5% simvastatin/zein mixture increased to 0.402 P due to the presence of drug in zein solution. Also, the presence of the indicative peaks associated with simvastatin in the Fourier transform infrared (FTIR) spectra of the samples containing the drug, verifies the existence of the drug on the polymeric base of zein/polyethylene oxide (PEO). Also, around 53.887% of the loaded drug was released in the first 12 h of the release kinetics assay and the results of the MTT assay shows reduction in the number of live cells in the test sample. In conclusion, zein nanofibers containing simvastatin drug were found to be potential biodegradable polymers that can find application in bone tissue repair.
本研究旨在通过静电纺丝法制备并表征载有辛伐他汀的玉米醇溶蛋白纳米纤维，使用含有辛伐他汀抗生素的玉米醇溶蛋白溶液作为聚合物基质。研究了纤维的物理、细胞特性和药物释放动力学。通过实施扫描电子显微镜（SEM）研究纤维的形成。SEM 图像显示，由 1.5%辛伐他汀/玉米醇溶蛋白溶液制成的纤维具有均匀的无珠网络纤维结构。由于药物存在于玉米醇溶蛋白溶液中，1.5%辛伐他汀/玉米醇溶蛋白混合物的粘度增加到 0.402 P。此外，在含有药物的样品的傅里叶变换红外（FTIR）光谱中存在与辛伐他汀相关的特征峰，验证了药物存在于玉米醇溶蛋白/聚环氧乙烷（PEO）的聚合物基质上。此外，在释放动力学测定的前 12 小时内，约 53.887%的载药被释放，MTT 测定的结果表明测试样品中活细胞数量减少。总之，载有辛伐他汀药物的玉米醇溶蛋白纳米纤维被发现是潜在的可生物降解聚合物，可用于骨组织修复。
