Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430061, China.
Department of Integrated Traditional Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Huangshi No.5 Hospital, Huangshi 435005, China.
Chin J Nat Med. 2021 Apr;19(4):267-283. doi: 10.1016/S1875-5364(21)60029-8.
Ulcerative colitis (UC) is a chronic refractory non-specific intestinal inflammatory disease that is difficult to be cured. The discovery of new ulcerative colitis-related metabolite biomarkers may help further understand UC and facilitate early diagnosis. It may also provide a basis for explaining the mechanism of drug action in the treatment of UC. Compound Sophorae Decoction (CSD) is an empirical formula used in the clinical treatment of UC. Although it is known to be efficacious, its mechanism of action in the treatment of UC is unclear. The purpose of this study was to investigate the changes in endogenous substances in UC rats and the effects of CSD on metabolic pathways using the metabonomics approach. Metabolomics studies in rats with UC and normal rats were performed using LC-MS/MS. Rats with UC induced using TNBS enema were used as the study models. Metabolic profiling and pathway analysis of biomarkers was performed using statistical and pathway enrichment analyses. 36 screened potential biomarkers were found to be significantly different between the UC and the normal groups; it was also found that CSD could modulate the levels of these potential biomarkers. CSD was found to be efficacious in UC by regulating multiple metabolic pathways.
溃疡性结肠炎（UC）是一种难以治愈的慢性难治性非特异性肠道炎症性疾病。发现新的溃疡性结肠炎相关代谢物生物标志物可能有助于进一步了解 UC 并促进早期诊断。它也可能为解释 UC 治疗中药物作用机制提供依据。复方苦参汤（CSD）是一种用于临床治疗 UC 的经验方。虽然已知其有效，但 CSB 在治疗 UC 中的作用机制尚不清楚。本研究旨在采用代谢组学方法研究 UC 大鼠内源性物质的变化及 CSD 对代谢途径的影响。采用 LC-MS/MS 对 UC 大鼠和正常大鼠进行代谢组学研究。采用 TNBS 灌肠法诱导 UC 大鼠作为研究模型。采用统计和途径富集分析对生物标志物进行代谢谱和途径分析。在 UC 组和正常组之间发现 36 个筛选出的潜在生物标志物存在显著差异；还发现 CSD 可以调节这些潜在生物标志物的水平。CSD 通过调节多种代谢途径对 UC 有效。
