Department of Plastic Surgery, Xiangya Hospital, Central South University, Changsha, 410011, Hunan Province, P. R. China.
Department of Burns and Reconstructive Surgery, Xiangya Hospital, Central South University, Changsha, 410011, Hunan Province, P. R. China.
Cell Death Dis. 2021 Apr 19;12(5):410. doi: 10.1038/s41419-021-03612-z.
Recently studies found that APEX1 was abnormally expressed in melanoma, indicating that it might be involved in the development of melanoma. However, the underlying mechanism and the interaction between APEX1 and LINC00470 in melanoma are not clear. Therefore, we aimed to investigate the role of LINC00470 in the development of melanoma in this work. We discovered that LINC00470 was overexpressed in melanoma tissues and cells compared with the adjacent normal tissues and cells by qPCR. The overexpression of LINC00470 promoted the proliferation and migration of melanoma cells. The functional investigation demonstrated that LINC00470 activated the transcription factor, ZNF131, to regulate the APEX1 expression, which finally promoted cell proliferation and migration. In contrast, knockdown of LINC00470 could significantly inhibit the melanoma cell proliferation and migration, and suppress the growth of tumor in vivo. Overexpression of APEX1 could reverse the impact of the silence of LINC00470 in melanoma cells. In summary, our studies revealed that LINC00470 promoted melanoma proliferation and migration by enhancing the expression of APEX1, which indicated that LINC00470 might be a therapeutic target for the treatment of melanoma.
最近的研究发现，APEX1 在黑色素瘤中异常表达，表明它可能参与了黑色素瘤的发生发展。然而，APEX1 在黑色素瘤中的作用机制及其与 LINC00470 的相互作用尚不清楚。因此，我们旨在研究 LINC00470 在黑色素瘤发展中的作用。通过 qPCR 我们发现，与相邻正常组织和细胞相比，LINC00470 在黑色素瘤组织和细胞中呈过表达。LINC00470 的过表达促进了黑色素瘤细胞的增殖和迁移。功能研究表明，LINC00470 激活转录因子 ZNF131 来调节 APEX1 的表达，最终促进细胞增殖和迁移。相反，LINC00470 的敲低可显著抑制黑色素瘤细胞的增殖和迁移，并抑制体内肿瘤的生长。APEX1 的过表达可逆转 LINC00470 沉默对黑色素瘤细胞的影响。综上所述，我们的研究表明，LINC00470 通过增强 APEX1 的表达促进黑色素瘤的增殖和迁移，这表明 LINC00470 可能是治疗黑色素瘤的一个潜在靶点。
